2023-06-07T01:23:01Z
ENA-FIRST-PUBLIC
2023-06-07T14:35:26Z
ENA-LAST-UPDATE
GSM6636913
External Id
SAMN31271708
External Id
2023-06-07T01:23:01Z
INSDC first public
2023-06-07T14:35:26Z
INSDC last update
public
INSDC status
SRS15396746
SRA accession
Ring (4hpi)
Stage
GSM6636913
Submitter Id
NCBI
broker name
H3K4me2
chip antibody
missing
collection_date
missing
geo_loc_name
3D7
isolate
Cultures were synchronized to the ring stage by two sorbitol treatments then cultured for 25 hours in the presence of heparin (20 U/ml) to prevent reinvasion of erythrocytes. Once the majority of parasites had developed to the late schizont stage (fully segmented schizonts as assessed by Giemsa stain), the culture was centrifuged at 250 × g for 5 min. at RT to pellet the erythrocytes. The supernatant containing heparin was removed, and the erythrocytes resuspended in complete culture medium (5% hematocrit). The culture was incubated under normal growth conditions (37°C in an atmosphere of 5% O2, 5% CO2, and 90% N2) for 4 hours to allow reinvasion. Following the reinvasion window, mature parasites were removed from the culture by treatment with 5% sorbitol. To ensure complete removal of mature parasites, remaining trophozoite- and schizont-infected erythrocytes were removed by magnetic separation using a MidiMACS separator. Isolated ring stage (4 hpi) parasites were released from erythrocytes by saponin treatment.
isolation
Plasmodium falciparum 3D7
organism
Plasmodium falciparum 3D7
scientific_name
3D7
source_name
ChIP-seq of Plasmodium blood stages: R H3K4me2
title