MIMS.me
BioSampleModel
MIGS/MIMS/MIMARKS.soil
BioSampleModel
N
Blank
N
ConnectivityStudy
2023-06-08T01:20:31Z
ENA-FIRST-PUBLIC
2023-06-08T01:20:31Z
ENA-LAST-UPDATE
SAMN35625557
External Id
N
ExtrBlank
CTAGAGCT
Fwd_Primer_Barcode
AO
Group2
16
GroupOrAgit
pda|jaimiewest@orcid
INSDC center name
2023-06-08T01:20:31Z
INSDC first public
2023-06-08T01:20:31Z
INSDC last update
public
INSDC status
N
IncubBlank
N
Initial
N
Meta
NA
MetaOrder
Y
MixingStudy
Unsaturated
Moisture
Oxic
O2
3
Rep
GTCGTAGT
Rev_Primer_Barcode
SRS17928399
SRA accession
239_16X_O
Submitter Id
16X
TimesMixed
16X_O
Trt
N
VortexControl
N
WaterBlank
840 mm
annual_precpt
5 C
annual_temp
NCBI
broker name
2019-09-16
collection_date
soil core
collection_method
hardwoods (e.g. oak,hickory,elm,aspen)
cur_land_use
northern mesic forest, early-to-mid seral
cur_vegetation
15-20 cm
depth
moderately well
drainage_class
430 m
elev
woodland area[ENVO:00000109]
env_broad_scale
woodland area[ENVO:00000109]
env_local_scale
soil
env_material
16 week lab incubation
extreme_event
Luvisols
fao_class
USA:Wisconsin
geo_loc_name
45.748 N 90.731 W
lat_lon
Freeon silt loam
local_class
USDA soil classification
local_class_meth
Qiagen DNeasy PowerLyzer PowerSoil DNA extraction kit
nucl_acid_ext
soil metagenome
organism
initial denaturation:98_2; denaturation:98_0.167; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
pcr_cond
NA
ph
NA
ph_meth
not pooled
pool_dna_extracts
Metal core, 1.8 cm dia
samp_collect_device
4 C until sieving to 2mm and start of incubation at RT
samp_mat_process
50 mg
samp_size
50 mg
samp_vol_we_dna_ext
soil metagenome
scientific_name
Illumina MiSeq 2x250 PE
seq_meth
2mm
sieving
A horizon
soil_horizon
silt loam
soil_text_measure
Freeon silt loam
soil_type
USDA soil classification
soil_type_meth
after incubation, samples for DNA extraction were stored at -80 C for up to 1 month prior to DNA extraction
store_cond
16S rRNA
target_gene
V4
target_subfragment
239_16X_O
title