2024-08-17
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114316815
External Id
Istituto Nazionale di Genetica Molecolare (INGM)
INSDC center name
2023-08-30T17:53:35Z
INSDC last update
public
INSDC status
ERS16305929
SRA accession
E-MTAB-13319:Sample 1
Submitter Id
ArrayExpress
broker name
MEF cell line
cell_line
fibroblast
cell_type
not collected
collection date
house mouse
common name
Protocols: Immortalized MEF cells were treated with 100ug/ml cyclohexamide for 2 minutes then washed once with ice-cold PBS 10ug/ml cyclohexamide. Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cyclohexamide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028). Immagina Biotechnology LACEseq #LS-001_12
description
embryo
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
embryo
organism part
Mus musculus
scientific_name
Sample 1
title