2024-08-19
ENA first public
ERC000011
ENA-CHECKLIST
Molecular Cell Biology
INSDC center name
public
INSDC status
ERS20619978
SRA accession
E-MTAB-14292:Sample 12
Submitter Id
ArrayExpress
broker name
HepG2
cell_line
hepatocyte
cell_type
not collected
collection date
human
common name
Protocols: cells were pelleted using 0.25 % trypsin-EDTA for detaching, followed by washing with PBS and centrifugation at 2370 x g and 4 °C for 4 min.  After removing the supernatant, total RNA of the cell pellet was isolated using RNeasy Plus Mini Kit which includes DNAse digestion (Qiagen).  Poly-A RNA libraries were prepared using the D-Plex mRNA-seq Kit for Illumina (Hologic Diagenode)
description
adult
developmental stage
liver cancer
disease
HepG2
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
liver
organism part
Homo sapiens
scientific_name
untreated
stimulus
Sample 12
title