2024-08-22
ENA first public
ERC000011
ENA-CHECKLIST
Zhengzhou Central Hospital Affiliated To Zhengzhou University
INSDC center name
public
INSDC status
ERS20876509
SRA accession
E-MTAB-14351:A15
Submitter Id
ArrayExpress
broker name
T1
clinical history
not collected
collection date
human
common name
Protocols: Patients with ICH who were admitted to the emergency department and non-hospitalized individuals who underwent health checkups during the same period were recruited in this study from May to September 2023. All participants were local residents of Henan Province. The study patients with ICH met the following inclusion criteria: age 18–75 years, admission 24 h after ICH, ICH confirmed by cranial computed tomography, and underwent hematoma evacuation; exclusion criteria included traumatic ICH, preoperative liver and kidney dysfunction, history of previous gastrointestinal surgery, history of gastrointestinal diseases, history of immune-related diseases or being given immunotherapy, receiving antibiotics or probiotics one month before onset, and death within 72 h after surgery. Patients with ICH who were admitted to the emergency department and non-hospitalized individuals who underwent health checkups during the same period were recruited in this study from May to September 2023. All participants were local residents of Henan Province. The study patients with ICH met the following inclusion criteria: age 18–75 years, admission 24 h after ICH, ICH confirmed by cranial computed tomography, and underwent hematoma evacuation; exclusion criteria included traumatic ICH, preoperative liver and kidney dysfunction, history of previous gastrointestinal surgery, history of gastrointestinal diseases, history of immune-related diseases or being given immunotherapy, receiving antibiotics or probiotics one month before onset, and death within 72 h after surgery.  Total community genomic DNA was extracted using an E.Z.N.A™ MagBind Soil DNA Kit (Omega, M5635-02). Purity was determined using a Qubit 4.0 (Thermo, USA), and high-quality DNA was amplified using the 341F (5′-CCTACGGGNGGCWGCAG-3′) and 805R (5′-GACTACHVGGGTATCTAATCC-3′) primers in the hypervariable V3–V4 region of the bacterial 16S rRNA gene. PCR mixtures were prepared with 1 µL of each primer and 2 µL of template DNA; 2×Hieff® Robust PCR Master Mix (Yeasen, 10105ES03, China) was added to a final volume of 30 µL. The plate was sealed, and PCR was performed using a thermal instrument (Applied Biosystems 9700, USA).  Total community genomic DNA was extracted using an E.Z.N.A™ MagBind Soil DNA Kit (Omega, M5635-02). Purity was determined using a Qubit 4.0 (Thermo, USA), and high-quality DNA was amplified using the 341F (5′-CCTACGGGNGGCWGCAG-3′) and 805R (5′-GACTACHVGGGTATCTAATCC-3′) primers in the hypervariable V3–V4 region of the bacterial 16S rRNA gene. PCR mixtures were prepared with 1 µL of each primer and 2 µL of template DNA; 2×Hieff® Robust PCR Master Mix (Yeasen, 10105ES03, China) was added to a final volume of 30 µL. The plate was sealed, and PCR was performed using a thermal instrument (Applied Biosystems 9700, USA).
description
intracerebral hemorrhage (ICH)
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
feces
organism substance
human gut metagenome
sample type
Homo sapiens
scientific_name
A15
title