2024-08-23
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114188865
External Id
Barts Cancer Institute
INSDC center name
2023-07-24T09:28:02Z
INSDC last update
public
INSDC status
ERS16169561
SRA accession
E-MTAB-13174:Sample 1
Submitter Id
ArrayExpress
broker name
MV-4-11
cell_line
not collected
collection date
human
common name
Protocols: MV-4-11 cells were plated at density 200 000/mL and treated with agents tested for 24h. Before RNA isolation cells were washed in cold PBS. The RNA was isolated using Direct-zol RNA Miniprep kit (Zymo Research) following the manufacturer’s protocol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. The clustering of the index-coded samples was performed according to the manufacturer’s instructions.
description
juvenile stage
developmental stage
biphenotypic B-myelomonocytic leukemia
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
Sample 1
title