2024-08-26
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA111457575
External Id
UNIVERSITY OF MANCHESTER
INSDC center name
2022-10-12T09:45:07Z
INSDC last update
public
INSDC status
ERS13551611
SRA accession
E-MTAB-12142:differentiated lung organoid_2
Submitter Id
ArrayExpress
broker name
mixed SOJD3, SEUR7, WT02C3 and WT02C9
cell_line
iPSC differentiated lung organoids
cell_type
human
common name
Protocols: 4 iPSC lines used are: SOJD3, SEUR7, WT02C3 and WT02C9. nto anterior foregut endoderm and mesoderm progenitors. The two population of cells were imbedded in Matrigel and co-cultured in an insert of a 12 mm Transwell that was emerged in DMEM base medium supplemented with human serum replacement 3, BMP4, FGF2, FGF7, and FGF10. The medium was changes every 2-3 days and the pulmonary organoids spontaneously formed within 14 days of the co-culture. The pulmonary organoids were first treated with Corning cell recovery solution (Corning, Deeside, UK) to recover cells before nucleic RNA extraction. The induced pluripotent stem cells were cultured in Essential 8 Medium (Thermo Fisher Scientific) The pulmonary organoids were first treated with Corning cell recovery solution (Corning, Deeside, UK) to recover cells, and then the total RNAs were extracted using TRIZOL and phenol-chloroform. Total RNA from iPSCs was extracted using the RNeasy Mini Kit (Qiagen). Illumina TruSeq Stranded mRNA kit, according to the manufacturer’s protocol.
description
embryo
developmental stage
normal
disease
wild type genotype
genotype
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
skin
organism part
induced pluripotent stem cell
progenitor cell type
Homo sapiens
scientific_name
differentiated lung organoid_2
title