2024-08-26
ENA first public
ERC000011
ENA-CHECKLIST
MPUSP
INSDC center name
public
INSDC status
ERS20622930
SRA accession
iclip_yebc_uv_1
Submitter Id
Max Planck Unit for the Science  of Pathogens
collected_by
2022-09-14
collection date
The strain yebC:3xFLAG was grown in 400 mL of chemically defined medium until the mid-logarithmic growth phase. The cells were rapidly cooled on ice-water bath and collected by centrifugation for 5 min at 4500 g +4°C. The cells were re-suspended in 100 mL of ice-cold PBS, transferred to cooled trays and UV irradiated with 600 mJ cm-2 UV-C. The cells were were collected by centrifugation for 5 min at 4500 g +4°C.
description
Germany
geographic location (country and/or sea)
Streptococcus pyogenes M1 GAS
organism
Streptococcus pyogenes M1 GAS
scientific_name
M1
serotype
SF370
strain
yebC:3xFLAG (EC3640)
sub_strain
iCLIP for yebC:3xFLAG strain UV+ replicate 1
title