2024-08-26
ENA first public
ERC000011
ENA-CHECKLIST
MPUSP
INSDC center name
public
INSDC status
ERS20622933
SRA accession
iclip_wt_uv
Submitter Id
Max Planck Unit for the Science  of Pathogens
collected_by
2022-09-15
collection date
The WT strain was grown in 400 mL of chemically defined medium until the mid-logarithmic growth phase. The cells were rapidly cooled on ice-water bath and collected by centrifugation for 5 min at 4500 g +4°C. The cells were re-suspended in 100 mL of ice-cold PBS, transferred to cooled trays and UV irradiated with 600 mJ cm-2 UV-C. The cells were were collected by centrifugation for 5 min at 4500 g +4°C and stored at -80°C.
description
Germany
geographic location (country and/or sea)
Streptococcus pyogenes M1 GAS
organism
Streptococcus pyogenes M1 GAS
scientific_name
M1
serotype
SF370
strain
WT (EC2224)
sub_strain
iCLIP for WT strain UV+
title