2024-08-28
ENA first public
ERC000011
ENA-CHECKLIST
University of Pisa
INSDC center name
public
INSDC status
ERS20922642
SRA accession
E-MTAB-14403:Sample 17
Submitter Id
60
day
age
1612_HP_WT
assay name
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: hippocampus dissection from experimental animals Transplantation of neural precursor cells (NPCs, 10 million of cells) or PBS (10 ul) intra-cisterna magna, under anaesthesia RNA was extracted using Purezol (cod.7326890 Biorad). DNasi I amplification Grade (cod.AMPD1, Sigma-Aldrich) was used to remove possible genomic DNA contamination and a second RNA extraction was performed with Purezol. RNA integrity was verified using the RNA 6000 nano Kit on a Bioanalizer (Agilent Technologies). All samples had a RIN>9. RNA was quantified using a Qubit RNA BR Assay Kit. Libraries were prepared according to the following reference guide: /https://emea.support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/illumina_prep/RNA/illumina-stranded-mrna-reference-guide-1000000124518-02.pdf
description
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
hippocampus
organism part
Mus musculus
scientific_name
male
sex
CD1
strain
Sample 17
title
no treatment
treatment