2024-08-28
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112843717
External Id
Dept.Biometra, University of Milan
INSDC center name
2023-03-28T11:32:31Z
INSDC last update
public
INSDC status
ERS14850326
SRA accession
E-MTAB-12813:1600_KO
Submitter Id
60
day
age
ArrayExpress
broker name
house mouse
common name
Protocols: cerebellum dissection from experimental animals Transplantation of neural precursor Cells (NPCs, 10 million of cells) or PSB (10ul) intra-cisterna magna, under anaesthesia RNA was extracted by using Purezol (cod.7326890; Bio-rad) following . DNasi I Amplification Grade (cod.AMPD1; Sigma-Aldrich) was used to remove eventual genomic DNA contamination and a second RNA extraction was performed in Purezol. RNA integrity was verified using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies); all samples had a RIN>9. RNA was quantified using Qubit RNA BR Assay Kit. Libraries were prepared accordingly to the following guides: https://emea.support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/illumina_prep/RNA/illumina-stranded-mrna-reference-guide-1000000124518-02.pdf
description
normal
disease
Mecp2 KO
genotype
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
cerebellum
organism part
Mus musculus
scientific_name
male
sex
CD1
strain
1600_KO
title