2024-08-29
ENA first public
ERC000011
ENA-CHECKLIST
INRAE
INSDC center name
public
INSDC status
ERS20599472
SRA accession
E-MTAB-14261:GF 3
Submitter Id
21
day
age
ArrayExpress
broker name
not collected
collection date
chicken
common name
Protocols: At day 21 post-hatching, CV and GF birds (n=8 per group) were sacrificed by cervical dislocation followed by blood withdrawal from the occipital venous plexus. Blood samples were stored in heparin-coated tubes for subsequent plasma preparation. Lung samples were immediately collected from each chicken, snap frozen in liquid nitrogen and stored at -80°C until further use for RNA extraction. Total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Germany) with rDNAse treatment to eliminate contaminant DNA. RNA quality was checked by the Bioanalyzer 2100 system using the RNA Nano 6000 Assay kit (Agilent Technologies, CA, USA).  The NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® was used to prepare the library. RNA with PolyA selection.
description
adult
developmental stage
B21/B21
genotype
not collected
geographic location (country and/or sea)
GF 3
individual
not applicable
isolate
Gallus gallus
organism
Gallus gallus
organism
lung
organism part
Gallus gallus
scientific_name
undefined
sex
White leghorn PA12
strain
GF 3
title