2024-08-29
ENA first public
ERC000011
ENA-CHECKLIST
INRAE
INSDC center name
public
INSDC status
ERS20599500
SRA accession
E-MTAB-14270:20 H CC
Submitter Id
14
day
age
ArrayExpress
broker name
not collected
collection date
chicken
common name
Protocols: At days 7, 14 and 21 post-hatching conventional (CV) chickens were sacrificed by cervical dislocation followed by blood withdrawal from the occipital venous plexus. Caecal contents were immediately collected from each chicken, snap frozen in liquid nitrogen and stored at -80°C until further use for DNA extraction and 16S sequencing.   Total DNA was extracted from caecal contents using the Dneasy PowerSoil kit (Qiagen, UK) following the manufacturer’s protocol. 16S (V3-V4 region) amplification was performed as described by Zheng et al (An accurate and efficient experimental approach for characterization of the complex oral microbiota. Microbiome 3, 48, 2015). In the limited cycle 2nd round PCR, sample-specific barcodes were added for multiplexing.
description
B21/B21
genotype
not collected
geographic location (country and/or sea)
CV 12
individual
not applicable
isolate
Gallus gallus
organism
Gallus gallus
organism
caecal contents
organism part
Gallus gallus
scientific_name
White leghorn PA12
strain
20 H CC
title