2024-08-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112773396
External Id
Medical School of Nantong University
INSDC center name
2023-03-15T11:58:01Z
INSDC last update
public
INSDC status
ERS14774193
SRA accession
E-MTAB-12764:Sample 9
Submitter Id
ArrayExpress
broker name
hepatic stellate cell
cell_type
Norway rat
common name
Protocols: Hepatic stellate cells were obtained by a two-step enzyme digestion protocol and further purified by magnetic-activated cell sorting (MACS) negative selection (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) to remove possible contamination of CD45+ myeloid cells and CD31+ endothelial cells according to the manufacturer's instructions. Hepatic stellate cells derived sEVs were obtained using an ExoQuick kit (System Biosciences, Inc.). TRIzol (Life Technologies) Illumina TruSeq Small RNA Sample kit (Illumina)
description
adult
developmental stage
extracellular vesicle
fraction
wild type genotype
genotype
not applicable
isolate
Rattus norvegicus
organism
Rattus norvegicus
organism
liver
organism part
activated
phenotype
Rattus norvegicus
scientific_name
male
sex
Sprague Dawley
strain
7 to 14
day
time
Sample 9
title
450-550
gram
weight