2024-08-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115120763
External Id
Fondazione Istituto Nazionale di Genetica Molecolare (INGM)
INSDC center name
2024-01-19T16:54:21Z
INSDC last update
public
INSDC status
ERS17768505
SRA accession
E-MTAB-13718:Adult CD4+ Naive LINE1 replicate 2
Submitter Id
ArrayExpress
broker name
naive CD4+ T cell
cell_type
not collected
collection date
human
common name
Protocols: Naïve CD4+ T cells have been isolated from PBMCs (umbilical for neonatal t cells and peripheral for adult t cells) by density gradient centrifugation with Ficoll-Paque Plus. From PBMCs Naive CD4+ were FACS sorted as CD4+ CD45RA+ CD45RO- Adult naive CD4+ T cells were cultured with LINE1 or scramble (control) ASOs RNA has been extracted with Maxwell RCS simplyRNA Cells kit Libraries were prepared using Illumina Stranded mRNA prep kit and following manufacturer’s guidelines
description
adult
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
CD4+ CD45RA+ CD45RO-
immunophenotype
2
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
Adult CD4+ Naive LINE1 replicate 2
title