2024-08-31
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114514185
External Id
Institute of Human Genetics Polish Academy of Sciences
INSDC center name
2023-10-18T18:52:09Z
INSDC last update
public
INSDC status
ERS16495531
SRA accession
E-MTAB-13462:Jur_143_1_ip
Submitter Id
ArrayExpress
broker name
Jurkat
cell_line
lymphoblast
cell_type
not collected
collection date
human
common name
Protocols: For each immunoprecipitation reaction 30 million transduced cells were used. The Ago2-RIP was performed using Magna RIP Kit (Merck) and anti-Ago2 antibody (Abnova, H00027161-M01). For total RNA isolation 3 million cultured Jurkat T-ALL cells were harvested and rinsed twice with phosphate buffered saline. Total RNA was isolated using miRNeasy Kit (Qiagen). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). For each immunoprecipitation reaction 30 million transduced cells were used. The Ago2-RIP was performed using Magna RIP Kit (Merck) and anti-Ago2 antibody (Abnova, H00027161-M01). For total RNA isolation 3 million cultured Jurkat T-ALL cells were harvested and rinsed twice with phosphate buffered saline.
description
juvenile
developmental stage
T-cell acute lymphoblastic leukemia
disease
pCDH_CMV_MCS_EF1_GFP_puro pre-mir-143
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
1
replicate
Homo sapiens
scientific_name
Jur_143_1_ip
title