2024-09-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114411287
External Id
University of Cambridge
INSDC center name
2023-09-22T11:38:38Z
INSDC last update
public
INSDC status
ERS16399951
SRA accession
E-MTAB-13366:Valproate_S11
Submitter Id
ArrayExpress
broker name
CD8-positive, alpha-beta T cell
cell_type
not collected
collection date
human
common name
Protocols: CD8+ T cells were isolated by positive magnetic selection from leucocyte cones from healthy blood donors before being stimulated with anti-CD2/3/28 microbeads in complete RPMI medium with non-limiting IL2 (10ng/ml) in the presence of either sodium valproate (500uM), vehicle (1 in 10,000 DMSO) or sodium valproate (175uM) with concurrent glutaminolysis inhibition (R162, 25uM) Total RNA was extracted from harvested cell populations using an RNeasy mini kit (Qiagen) with quality assessed using an Agilent BioAnalyser 2100 and RNA quantification performed using a NanoDrop ND-1000 spectrophotometer. Library preparation was undertaken using the SMARTer Stranded Total RNA-seq v2 Pico Input Mammalian kit according to the manufacturer's instructions
description
normal
disease
not collected
geographic location (country and/or sea)
CD8+
immunophenotype
S11
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
Valproate_S11
title