2024-09-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114411408
External Id
University of Cambridge
INSDC center name
2023-09-22T12:36:22Z
INSDC last update
public
INSDC status
ERS16400072
SRA accession
E-MTAB-13368:Sample 9
Submitter Id
6
week
age
ArrayExpress
broker name
T cell
cell_type
not collected
collection date
house mouse
common name
Protocols: CD3+44+8+ T cells were isolated from splenocytes on day 5 after restimulation with a heterotypic strain of influenza, having received primary infection 30 days previously along with either sodium valproate (0.1%w/v) in drinking water or vehicle (1 in 10,000 DMSO). Following CD8+ T cell isolation as above, 50,000 cells were lysed (1M Tris-HCl) and the nuclear pellet transposed using the Tn5 transposase (TDE1, Illumina) at 37C for 30min before DNA isolation (Qiagen minElute) and PCR amplification (NEBnext) guided by qPCR (KAPA-SYBR) estimation of reporter signal v cycle number and PCR cycle length tailored to 1/3 maximal amplification Libraries were purified (AMPure XP) with quality assessed using an Agilent BioAnalyser 2100 and RNA quantification performed using a NanoDrop ND-1000 spectrophotometer.
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
CD44+8+
immunophenotype
S8
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
spleen
organism part
Mus musculus
scientific_name
C57BL/6
strain
Sample 9
title