2024-09-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114411344
External Id
University of Cambridge
INSDC center name
2023-09-22T12:21:09Z
INSDC last update
public
INSDC status
ERS16400008
SRA accession
E-MTAB-13367:Sample 9
Submitter Id
ArrayExpress
broker name
CD8-positive, alpha-beta T cell
cell_type
not collected
collection date
human
common name
Protocols: CD8+ T cells were isolated by positive magnetic selection from leucocyte cones from healthy blood donors before being stimulated with anti-CD2/3/28 microbeads in complete RPMI medium with non-limiting IL2 (10ng/ml) in the presence of either sodium valproate (175uM) or vehicle (1 in 10,000 DMSO) Following CD8+ T cell culture as above, 50,000 cells were lysed (1M Tris-HCl) and the nuclear pellet transposed using the Tn5 transposase (TDE1, Illumina) at 37C for 30min before DNA isolation (Qiagen minElute) and PCR amplification (NEBnext) guided by qPCR (KAPA-SYBR) estimation of reporter signal v cycle number and PCR cycle length tailored to 1/3 maximal amplification Libraries were purified (AMPure XP) with quality assessed using an Agilent BioAnalyser 2100 and RNA quantification performed using a NanoDrop ND-1000 spectrophotometer.
description
adult
developmental stage
normal
disease
not collected
geographic location (country and/or sea)
CD8+
immunophenotype
S13
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
Sample 9
title