2024-09-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114396335
External Id
University of Cambridge
INSDC center name
2023-09-18T21:01:25Z
INSDC last update
public
INSDC status
ERS16385398
SRA accession
E-MTAB-13353:P3_IR_2
Submitter Id
ArrayExpress
broker name
Nestin-expressing progenitor
cell_type
not collected
collection date
house mouse
common name
Protocols: The single-cell RNA-Seq of FACS-sorted cell suspensions was performed on Chromium instrument (10X genomics) following the user guide manual for 3′ v3.1. In brief, FACS-sorted cells from different conditions were multiplexed using CellPlex reagents (10x Genomics) as described by the manufacturers protocol. The viability of cells was above 80%, as confirmed with 0.2% (w/v) Trypan Blue staining and barcoded cells were pooled into a single sample in PBS containing 1% BSA to a final concentration of 700–1,300 cells per μl. Between 2,000 to 3,000 cells were targeted for each sample. Samples were multiplexed together on one lane of 10X Chromium following 10x Genomics 3’ CellPlex Multiplexing protocol and a total of ~30,000 cells were targeted for droplet formation. Following reverse transcription and cell barcoding in droplets, emulsions were broken, and cDNA purified using Dynabeads MyOne SILANE followed by PCR amplification per manufacturer’s instruction.
description
P3
developmental stage
Nes-CFP/+
genotype
not collected
geographic location (country and/or sea)
CFP+
immunophenotype
brain injury
injury
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
cerebellum
organism part
Mus musculus
scientific_name
male
sex
Nes-Cfp
strain
P3_IR_2
title