2024-09-01
ENA first public
ERC000011
ENA-CHECKLIST
ITQB NOVA
INSDC center name
public
INSDC status
ERS20619918
SRA accession
E-MTAB-14289:SLR1_control_rep2
Submitter Id
14
day
age
ArrayExpress
broker name
not collected
collection date
Asian cultivated rice
common name
Nipponbare
cultivar
Protocols: Shoots from 14-day seedlings of Nipponbare, SLR1-OX #2, and SUMO1SLR1-OX #2 in control and subjected to salt stress for 8h (NaCl 120 mM) were collected and frozen in liquid nitrogen. Dehusked rice seeds were surface sterilized with 70% ethanol for 10 min and further sterilized with 25% bleach (commercial bleach) for 15 min, then washed five times in sterile water. The seeds were placed in hydroponics with half-strength Murashige & Skoog Medium and MES buffer 250 mg/L (Duchefa) with pH adjusted to 5.2. Seedlings were grown for 14 days before the salt stress assay. Then, the seedlings were exposed to salt stress for 8h (NaCl 120 mM). Dehusked rice seeds were surface sterilized with 70% ethanol for 10 min and further sterilized with 25% bleach (commercial bleach) for 15 min, then washed five times in sterile water. The seeds were placed in hydroponics with half-strength Murashige & Skoog Medium and MES buffer 250 mg/L (Duchefa) with pH adjusted to 5.2. Seedlings were grown for 14 days before the salt stress assay. Then, the seedlings were exposed to salt stress for 8h (NaCl 120 mM). RNA was extracted from a total of 100 mg of ground shoot sample with a Direct-zol kit (Zymo Research) according to the manufacturer’s protocol with in-column DNAse treatment. The cDNA libraries were obtained from 10 ng total RNA and using an optimized SMARTSeq2 protocol.
description
leaf development stage
developmental stage
control
environmental stress
SLR1-OX #2
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Oryza sativa
organism
Oryza sativa
organism
shoot system - leaf
organism part
2
replicate
Oryza sativa
scientific_name
SLR1_control_rep2
title