2024-09-01
ENA first public
ERC000011
ENA-CHECKLIST
Department Ecotoxicogenomics, Fraunhofer Institute for molecular biology and applied ecology (IME)
INSDC center name
public
INSDC status
ERS20275724
SRA accession
E-MTAB-14183:Sample 1
Submitter Id
96
hour
age
ArrayExpress
broker name
not collected
collection date
zebrafish
common name
3,3',5-triiodo-L-thyronine
compound
Protocols: at the end of exposure, 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep-24 homogeniser (MP Biomedicals, Irvine, USA) at 5 m/s speed for 45 seconds according to the RNA extraction protocol provided by the manufacturer (NucleoSpin RNA Protein kit; Macherey-Nagel 740933.250). Zebrafish embryos were exposed to T3 for 96 hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to 33 µg/L (mid exposure concentration) of T3 (3, 3′,5-Triiodo-L-thyronine, CAS 6893-02-3) in addition to untreated control groups.  At the end of exposure time (96 hours), RNA was extracted from 10 embryos using a NucleoSpin RNA/Protein kit (Macherey-Nagel, Düren, Germany). libraries were prepared at NGS-Services for Integrative Genomics at the University of Göttingen in Germany. For each 100 ng/µL sample, mRNA was purified through poly A selection followed by cDNA libraries preparation using the TruSeq RNA Library Prep Kit v2 (Illumina).
description
embryo stage
developmental stage
33
microgram per liter
dose
not collected
geographic location (country and/or sea)
not applicable
isolate
Danio rerio
organism
Danio rerio
organism
whole organism
organism part
Danio rerio
scientific_name
AB
strain
T14_3I
tank
Sample 1
title