SAMN37864592
External Id
National Center for Genetic Engineering and Biotechnology (BIOTEC), NSTDA
INSDC center name
2024-08-30T00:50:19Z
INSDC first public
2024-08-30T00:50:19Z
INSDC last update
SRS19183072
INSDC secondary accession
live
INSDC status
MIGS.eu
NCBI submission model
MIGS.eu.6.0
NCBI submission package
SRS19183072
SRA accession
not applicable
collection_date
Keywords: GSC:MIxS;MIGS:6.0
description
Intraerythrocytic mixed stages
dev_stage
not applicable
env_broad_scale
not applicable
env_local_scale
not applicable
env_medium
not applicable
geo_loc_name
Parasites were cultured using a standard method (Aroonsri et al., 2016). In brief, cultures contained Roswell Park Memorial Institute 1640 media (Gibco) made complete by supplementation with 0.5% (w/v) Albumax II, L-glutamine (Gibco), 0.2% (w/v) glucose, 25 mM HEPES, 25 mM NaHCO3, 50 g/mL hypoxanthine and 40 g/mL gentamicin. Leukocyte-depleted O+ type red blood cells were supplied at a final 2% hematocrit. The cultures were incubated at 37C in a gas mixture of 1% O2, 5% CO2 and 94% N2.
isol_growth_condt
Dd2_parent
isolate
not applicable
lat_lon
Plasmodium falciparum Dd2
http://purl.obolibrary.org/obo/NCBITaxon_57267
organism
asexual
propagation
Dd2
strain
Dd2_parent
title