2024-09-05
ENA first public
ERC000011
ENA-CHECKLIST
Roslin Institute, University of Edinburgh
INSDC center name
public
INSDC status
ERS20895453
SRA accession
E-MTAB-14384:55435 TSB
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Overnight TSB Staphylococcus aureus cultures were standardised to OD600 0.1 in TSB and OD600 0.25 in 50% Milk/TSB cultures in 5ml volumes. All samples were incubated at 37°C; 180 rpm for 2h before pelleting at 4863xg 5 min. The pellet was suspended in 200 µl TE buffer and transferred into a 96-well plate and centrifuged. The supernatant was removed and the pellets immediately frozen on dry ice containing 100% ethanol. The frozen pellets were briefly stored at -80°C before shipment to Genewiz, UK for RNA extraction and paired-end RNA sequencing. RNA was extracted from Staphylococcus aureus using homogenization in the tissue Lyzer II (Qiagen) with 0.1 mm beads and beating at 30 Hz. The RNeasy Plus Mini Kit from Qiagen was used for RNA extractions. Libraries were prepared by GENEWIZ - Enrichment, cDNA synthesis, and adapter ligation
description
ST97
genotype
not collected
geographic location (country and/or sea)
TSB
growth condition
not applicable
isolate
Staphylococcus aureus
organism
Staphylococcus aureus
organism
whole organism
organism part
Non-milk clotting
phenotype
Staphylococcus aureus
scientific_name
Human
strain
55435 TSB
title