2024-09-09
ENA first public
ERC000011
ENA-CHECKLIST
Genomix4life s.r.l
INSDC center name
public
INSDC status
ERS18372629
SRA accession
E-MTAB-13871:CTRL13
Submitter Id
22
year
age
ArrayExpress
broker name
peripheral blood mononuclear cells (PBMCs)
cell_type
not collected
collection date
human
common name
Protocols: This case-control study consists of a mRNA analysis on peripheral blood mononuclear cells (PBMCs) performed by RNA-seq in 25 participants of Sicilian ancestry: 12  patients and 13 age- and sex-matched healthy controls, all recruited at the Oasi Research InstituteIRCCS of Troina (Italy). Total RNA was extracted using Zymo Research, according to the manufacturer’s instructions. The RNA was transcribed to cDNA using oligo (dT) primers and the GeneAmp RNA PCR Core Kit (Applied Biosystems) and the cDNA was amplified via real-time PCR using Power- SYBR Green PCR Master Mix (Promega) on the 7500 Fast Real-Time PCR System (Applied Biosystems). RNA concentration and purity were evaluated using NanoDrop™ 2000/2000c (Thermo Fisher Scientific, Waltham, MA, USA), whereas sample integrity was analyzed using Tape Station 4200 (Agilent Technologies, Santa Clara, CA, USA) using an RNA Screen Tape Assay. Indexed libraries were prepared from 1 µg/ea purified RNA using the TruSeq Stranded mRNA (Illumina, San Diego, CA, USA) Library Prep Kit according to the manufacturer’s instructions. After the enrichment of mRNA using oligo dT magnetic beads and fragmentation, cDNA synthesis was performed, followed by adapter ligation and PCR amplification. For library quantifications, TapeStation 4200 (Agilent Technologies) was used. Indexed libraries were pooled in equimolar amounts, with a final concentration of 1.5 nM.
description
control
disease
not collected
geographic location (country and/or sea)
5
individual
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
blood
organism part
Homo sapiens
scientific_name
male
sex
CTRL13
title