2024-09-13
ENA first public
ERC000011
ENA-CHECKLIST
Mario Negri Institute for pharmacological research (IRCCS)
INSDC center name
public
INSDC status
ERS20585317
SRA accession
E-MTAB-14239:shEDA2R_AMAXA_REP1
Submitter Id
ArrayExpress
broker name
myoblast
cell_type
not collected
collection date
human
common name
Protocols: Before library preparation, RNA concentration was evaluated through Qubit™ RNA High Sensitivity Assay Kit (Invitrogen, Walthman, MA, USA) while RNA quality was established using 4200 Tapestation (Agilent Technologies, Santa Clara, CA, USA).  Protocol: miRNeasy Mini Kit. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing. TruSeq Stranded Total RNA Sample Preparation Guide. Following the TruSeq Stranded Total RNA protocol (Illumina, San Diego, CA, USA), at least 500 ng of RNA whose RIN value was between 7 and 9, were used for RNA sequencing.
description
shEDA2R_AMAXA
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Myoblasts
organism part
REP1
replicate
Homo sapiens
scientific_name
shEDA2R_AMAXA_REP1
title