5
nanogram per microliter
DNA
2024-09-14
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA111349834
External Id
Gastroenterology Unit IRCCS Casa Sollievo della Sofferenza Hospital
INSDC center name
2022-09-14T09:17:12Z
INSDC last update
public
INSDC status
ERS13447381
SRA accession
E-MTAB-12171:Sample 24
Submitter Id
8
week
age
ArrayExpress
broker name
Protocols: Fresh fecal pool were collected from each cage containing each group of mice treated with different condition and then stored at -80° DNA microbial extraction was performed using the QIAamp DNA Stool Mini Kit (Qiagen) starting from 250 µl of each sample according to the manufacturer’s protocol. After assessing DNA concentration and purity, samples were stored at -80° until processing. The V3–V4 hypervariable region of the bacterial 16S rRNA gene was amplified from total DNA according to the Illumina 16S Metagenomic Sequencing Library Preparation instructions, as previously described in Diamanti-Picchianti A, et al (Int J Mol Med ). In summary, the V3-V4 amplicon was obtained by PCR with universal primers, followed by purification. A second PCR was then performed to barcode the libraries using the Illumina dual-index system. Following a second step of purification, libraries were diluted to 4nM and pooled.
description
adult
developmental stage
KRAS G12D
genotype
not applicable
isolate
mouse gut metagenome
organism
mouse gut metagenome
organism
feces
organism part
mouse gut metagenome
scientific_name
female
sex
BALB/c
strain
Sample 24
title
PANC1 pancreatic cancer cells
xenograft