2024-09-16
ENA first public
ERC000011
ENA-CHECKLIST
Institute for Rare Diseases Research, Instituto de Salud Carlos III (ISCIII).
INSDC center name
public
INSDC status
ERS20964831
SRA accession
E-MTAB-14441:Wild type
Submitter Id
7
day
age
ArrayExpress
broker name
heart cells
cell_type
not collected
collection date
house mouse
common name
Protocols: Whole hearts from 7-day-old PD7 wild-type (WT) (Dhx36f/f;Tnnt2 wt/wt) and mutant (Dhx36f/f;Tnnt2 cre/wt) mice were dissected, chopped into several pieces, snap-frozen and stored in liquid nitrogen. On the day of the experiment, wild-type and mutant hearts were pooled (two each), and nuclei were isolated using the Chromium Nuclei Isolation Kit with RNAse inhibitor (10X Genomics; #PN-1000494). Nuclei were captured in the Chromium Controller (10X Genomics) and processed with the Chromium Next GEM single cell Multiome ATAC+Gene Expression (GEX) Kit (10X Genomics; #PN-1000285) ATAC and GEX libraries from the same pool of pre-amplified Tn5 transposed DNA/cDNA
description
PD7
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
2 hearts
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
heart
organism part
Mus musculus
scientific_name
mix
sex
C57bl/6-CD1
strain
Wild type
title