2024-09-16
ENA first public
ERC000011
ENA-CHECKLIST
Gehring Lab Institute for Genetics  University of Cologne
INSDC center name
public
INSDC status
ERS20852390
SRA accession
E-MTAB-14342:96
Submitter Id
ArrayExpress
broker name
HEK293
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: Cells were harvested for RNA extraction 48 hours post-transfection using RNATri (Bio&Sell). Human embryonic kidney 293 (HEK293) cells were seeded in 6-well plates and grown to 75% confluence in DMEM high glucose with 10% FBS.  1 µg plasmid of pCDNA3.1-HA-SARS-CoV2 N were transfected using lipofectamine 2000 (Invitrogen). Total RNA was extracted from RNATri (Bio&Sell) samples, followed by DNase I digestion to remove contaminating DNA. Final RNA concentration was adjusted to 250 ng/µL using RNase-free water. 3 independent samples were prepared for each condition. Libraries were prepared using the mRNA enrichment method at BGI Genomics.
description
late embryonic stage
developmental stage
normal
disease
none
genetic modification
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
kidney
organism part
3
replicate
Homo sapiens
scientific_name
female
sex
96
title