2024-09-16
ENA first public
ERC000011
ENA-CHECKLIST
Institute for Rare Diseases Research, Instituto de Salud Carlos III (ISCIII).
INSDC center name
public
INSDC status
ERS20964836
SRA accession
E-MTAB-14440:WT2
Submitter Id
21
day
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: whole hearts (including atria) were surgically isolated from 2- to 3-week-old WT or Dhx36myh6 mice. The isolated organs were individually submerged into 1 mL TRIsure buffer (Meridian Bioscience #BIO-38032), immediately snap-frozen in liquid N2, and stored at -80ºC. Tissue homogenization was performed using the MagNA lyser system (Roche). total RNA was isolated from 4-6 hearts per genotype. The RNA was then pooled and purified on Qiagen-RNA-clean columns (Qiagen #74204). Library construction was performed with TruSeq™ RNA Sample Prep Kit v2 (Illumina)
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
heart
organism part
Mus musculus
scientific_name
mix
sex
C57bl/6-CD1
strain
WT2
title