2024-09-19
ENA first public
ERC000011
ENA-CHECKLIST
Imperial College Research Fellow Department of Brain Sciences - Faculty of Medicine
INSDC center name
public
INSDC status
ERS20918204
SRA accession
E-MTAB-14336:Sample 1
Submitter Id
ArrayExpress
broker name
B cell derived cell line
cell_line
B cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Mouse islets were isolated using collagenase (Roche) digestion as previously described(22). Islets were allowed to recover overnight preceding SLIC-CAGE and miRNA RT-qPCR experiments in RPMI 1640, 10% fetal bovine serum (FBS), L-glutamine, and 11 mM glucose at 37°C with 95% O2/ 5% CO2.  RNA was extracted using Trizol (Thermo Fisher Scientific) following the manufacturer's instructions. MiRNA reverse transcription (RT) and qPCR was performed from 30ng RNA as previously described(23) with miRCURY LNA probes and miRCURY LNA RT and SYBR® Green PCR Kits.  Let-7d-3p and miR-573-3p (data not shown) were used as endogenous controls due to their stable expression in our system(23).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
islet of Langerhans
organism part
Mus musculus
scientific_name
Sample 1
title