2024-09-19
ENA first public
ERC000050
ENA-CHECKLIST
EMBL Heidelberg
INSDC center name
public
INSDC status
ERS21060770
SRA accession
spire_mag_01098608
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
megahit 1.2.9
assembly software
default
binning parameters
metabat 2.12.1
binning software
human digestive system
broad-scale environmental context
EBI-EMG
broker name
2011-11
collection date
99.91
%
completeness score
checkm2 0.1.3
completeness software
1.97
%
contamination score
This sample represents a Third Party Annotation (TPA) binned metagenome assembled from the metagenomic run SRR2155351 of study SRP062282.
description
stool
environmental medium
Germany
geographic location (country and/or sea)
missing: third party data
DD
geographic location (latitude)
missing: third party data
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
human gut metagenome
isolation_source
human gut
local environmental context
human gut metagenome
metagenomic source
Phocaeicola vulgatus
organism
To gain more insight into the interconnection between microbiota, obesity and diet, we analyzed the metagenome of 16 patients following a weight-loss program based on a very low calorie inulin-enriched formula diet. In this longitudinal study we used shotgun sequencing of stool DNA to look at taxonomic and functional changes at different phases of the program. In 2 years we sequenced fecal samples at six time points, the baseline (before intervention, = T0), after diet intervention, (3 months later: T3), after reintroduction phase (T6), and three time points at the maintenance phase i.e. month 12 (T12), month 18 (T18) and month 24 (T24). In parallel, clinical parameters were monitored in order to establish potential relationships between microbiota and obesity-associated-disease particularly hypertension, insulin resistance, fatty liver and the metabolic syndrome. Moreover we analyzed the marker potential of microbial parameters for weight loss maintenance success.
project name
SAMN03922504
sample derived from
Phocaeicola vulgatus
scientific_name
Illumina HiSeq 2500
sequencing method
multi-marker approach
taxonomic identity marker
binned metagenome: spire_mag_01098608
title