2024-09-19
ENA first public
ERC000050
ENA-CHECKLIST
EMBL Heidelberg
INSDC center name
public
INSDC status
ERS21058753
SRA accession
spire_mag_00967203
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
megahit 1.2.9
assembly software
default
binning parameters
metabat 2.12.1
binning software
human digestive system
broad-scale environmental context
EBI-EMG
broker name
2012
collection date
91.38
%
completeness score
checkm2 0.1.3
completeness software
7.0
%
contamination score
This sample represents a Third Party Annotation (TPA) binned metagenome assembled from the metagenomic run ERR3450861 of study ERP115445.
description
stool
environmental medium
United Kingdom
geographic location (country and/or sea)
51.50000000
DD
geographic location (latitude)
-0.12000000
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
human gut metagenome
isolation_source
human gut
local environmental context
human gut metagenome
metagenomic source
Phocaeicola vulgatus
organism
Nextera XT libraries were prepared manually following the manufacturers' protocol (Illumina, PN. 15031942). Briefly, samples were normalized to 0.2 ng/ml DNA material per library using a Quant-iT picogreen assay system (Life Technologies, PN. Q33120) on an AF2200 plate reader (Eppendorf), then fragmented and tagged via tagmentation. Amplification was performed by Veriti 96 well PCR (Applied Biosystems) followed by AMPure XP bead cleanup (Beckman Coulter, PN. A63880). Fragment size for all libraries was measured using a Labchip GX Touch HiSens. Sequencing was performed on an Illumina HiSeq 2500 using SBS kit V4 Chemistry, with a read length of 2x125 bp. Metadata on TwinsUK twin participants are available to bona fide researchers under managed access due to governance and ethical constraints. Raw data should be requested via our website (http://twinsuk.ac.uk/resources-for-researchers/access-our-data/) and requests are reviewed by the TwinsUK Resource Executive Committee (TREC) regularly.
project name
SAMEA5814070
sample derived from
Phocaeicola vulgatus
scientific_name
Illumina HiSeq 2500
sequencing method
multi-marker approach
taxonomic identity marker
binned metagenome: spire_mag_00967203
title