2024-09-20
ENA first public
ERC000050
ENA-CHECKLIST
EMBL Heidelberg
INSDC center name
public
INSDC status
ERS21064963
SRA accession
spire_mag_02696030
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
megahit 1.2.9
assembly software
default
binning parameters
metabat 2.12.1
binning software
human digestive system
broad-scale environmental context
EBI-EMG
broker name
missing: third party data
collection date
94.17
%
completeness score
checkm2 0.1.3
completeness software
0.09
%
contamination score
This sample represents a Third Party Annotation (TPA) binned metagenome assembled from the metagenomic run ERR4135141 of study ERP109777.
description
stool
environmental medium
not provided
geographic location (country and/or sea)
missing: third party data
DD
geographic location (latitude)
missing: third party data
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
human gut metagenome
isolation_source
human gut
local environmental context
human gut metagenome
metagenomic source
Phocaeicola vulgatus
organism
Objective: Despite the association of nonalcoholic fatty liver disease (NAFLD) with obesity, NAFLD is also found in non-obese individuals. Few studies have focused on the role of the gut microbiome in non-obese NAFLD. We aimed to compare gut microbiome profiles between non-obese and obese NAFLD subjects.Design: The gut microbiome profiles of 171 Asian subjects with biopsy-proven NAFLD and 31 metabolically healthy controls were analyzed using 16S rRNA sequencing. Study subjects were divided into non-obese (n=64) and obese (n=138) groups. Each group was classified into three subgroups according to fibrosis severity (stage, 0 vs. 1 vs. 2–4). The microbiome profiles were compared with clinical metadata, including metabolic traits and genetic variants.Results: Significant alterations in gut microbiota according to fibrosis severity were observed only in non-obese subjects. Specifically, Enterobacteriaceae, Veilloneallaceae, and other gram-negative bacteria were gradually enriched with increasing fibrosis severity. Ruminococcaceae, however, was depleted with significant fibrosis (≥F2). Significant associations between these bacteria and serum free fatty acid, adipose tissue insulin resistance, and glycosylated hemoglobin were found only in non-obese subjects. Apparent and strong co-occurring/co-excluding interactions were shown in non-obese subjects. Particularly, the addition of microbial abundance of Enterobacteriaceae, Veilloneallaceae, and Ruminococcaceae to body mass index and fibrosis 4 index showed 0.953 of area under the receiver-operating characteristic curvefor predicting significant fibrosis in non-obese NAFLD subjects.Conclusion: Our results demonstrated disparate compositions in gut microbiota between obese and non-obese Asian NAFLD subjects according to fibrosis severity, highlighting the pathogenic role of gut microbiota, especially in non-obese NAFLD.
project name
SAMEA6827974
sample derived from
Phocaeicola vulgatus
scientific_name
NextSeq 550
sequencing method
multi-marker approach
taxonomic identity marker
binned metagenome: spire_mag_02696030
title