2024-09-20
ENA first public
ERC000050
ENA-CHECKLIST
EMBL Heidelberg
INSDC center name
public
INSDC status
ERS21066865
SRA accession
spire_mag_02537340
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
megahit 1.2.9
assembly software
default
binning parameters
metabat 2.12.1
binning software
human digestive system
broad-scale environmental context
EBI-EMG
broker name
2017
collection date
90.81
%
completeness score
checkm2 0.1.3
completeness software
0.28
%
contamination score
This sample represents a Third Party Annotation (TPA) binned metagenome assembled from the metagenomic run ERR5883834 of study ERP128268.
description
stool
environmental medium
Netherlands
geographic location (country and/or sea)
52.29398138
DD
geographic location (latitude)
4.95841566
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
human gut metagenome
isolation_source
human gut
local environmental context
human gut metagenome
metagenomic source
Bacteroides uniformis
organism
Background: Recent studies demonstrate that a Mediterranean diet has beneficial metabolic effects in metabolic syndrome subjects. Since we have shown that fecal microbiota transplantation (FMT) from lean donors exerts beneficial effects on insulin-sensitivity, in the present trial we investigated the potential synergistic effects on insulin-sensitivity of combining Mediterranean diet with donor FMT in subjects with metabolic syndrome.Design: Twenty-four male subjects with metabolic syndrome were put on a Mediterranean diet and after a two-week run-in phase, subjects were randomized to either lean donor (n=12) or autologous (n=12) FMT. Changes in gut microbiota composition and bacterial strain engraftment after 2-week dietary regimens and 6 weeks post-FMT were primary endpoints. Secondary objectives were changes in glucose fluxes (both hepatic and peripheral insulin sensitivity), postprandial plasma incretin (GLP-1) levels, subcutaneous adipose tissue inflammation and plasma metabolites.Results: Consumption of the Mediterranean diet resulted in a reduction in body weight, HOMA-IR and lipid levels. However, no large synergistic effects of combining the diet with lean donor FMT were seen on gut microbiota diversity after 6 weeks. Although we did observe changes in specific bacterial species and plasma metabolites, no significant beneficial effects on glucose fluxes, postprandial incretins or subcutaneous adipose tissue inflammation were detected.Conclusions: In this small pilot randomized controlled trial, no synergistic beneficial metabolic effects of combining Mediterranean diet with lean donor FMT on glucose metabolism were achieved. However, we observed engraftment of specific bacterial species. Future trials are warranted to test the combination of other microbial interventions and diets in metabolic syndrome.
project name
SAMEA8690980
sample derived from
Bacteroides uniformis
scientific_name
Illumina NovaSeq 6000
sequencing method
multi-marker approach
taxonomic identity marker
binned metagenome: spire_mag_02537340
title