2024-09-20
ENA first public
ERC000050
ENA-CHECKLIST
EMBL Heidelberg
INSDC center name
public
INSDC status
ERS21064305
SRA accession
spire_mag_02381455
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
megahit 1.2.9
assembly software
default
binning parameters
metabat 2.12.1
binning software
human digestive system
broad-scale environmental context
EBI-EMG
broker name
not collected
collection date
97.65
%
completeness score
checkm2 0.1.3
completeness software
2.7
%
contamination score
This sample represents a Third Party Annotation (TPA) binned metagenome assembled from the metagenomic run SRR15051278 of study SRP327183.
description
stool
environmental medium
not provided
geographic location (country and/or sea)
missing: third party data
DD
geographic location (latitude)
missing: third party data
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
human gut metagenome
isolation_source
human gut
local environmental context
human gut metagenome
metagenomic source
Phocaeicola vulgatus
organism
Centenarians, who are living more than a century, represent the ultimate model of successful longevity associated with less susceptibility to aging-associated illness and chronic inflammation. The gut microbiota has been considered as a criticial determinant for human health and longevity. Here we show that centenarians (average 107 yo) have distinct gut microbiome enriched in microbes capable of generating unique secondary bile acids, include iso-,3-oxo, and iso-allo-lithocolic acid (LCA), as compared to elderly (85-89 yo) and young (21-55 yo) controls. In particular, iso-allo-LCA biosysnthesis pathway had not been defined. By screening of 68 bacterial isolates from a centenarian's faecal microbiota, we identified Paraceteroides merdae and Odoribacteraceae stains as effective producers of iso-all-LCA. Furthermore, we revealed that 5'-reductase (5AR) and 3'-hydroxysteroid dehydrogenase (3'HSDH) were responsible for the iso-allo-LCA generation by generating and testing mutant P. merdae strains. Iso-allo-LCA exerted the most potent antimicrobial effects among the test bile acid compounds, against gram-positive (but not gram-negative) multidrug-resistant pathogens, such as Clostridium difficile and vancomycin-resitant Enterococcus faecium. These findings suggest that specific bile acid metabolism may be involved in reducing the risk of acquiring pathobionts and their related diseases, thereby potentially contributing to longevity.
project name
SAMN20081883
sample derived from
Phocaeicola vulgatus
scientific_name
Illumina NovaSeq 6000
sequencing method
multi-marker approach
taxonomic identity marker
binned metagenome: spire_mag_02381455
title