2024-09-20
ENA first public
ERC000011
ENA-CHECKLIST
CNRS
INSDC center name
public
INSDC status
ERS20964015
SRA accession
E-MTAB-14449:WT-THY-Rep3
Submitter Id
ArrayExpress
broker name
M28PF1
cell_line
not collected
collection date
Protocols: GAS strains were cultured at 37 °C in THY or THY-Tween, and cells were harvested during exponential growth (OD600 between 0.4 and 0.5). Independent triplicate cultures were prepared for each condition.  For RNA preparation, 2 volumes of RNA protect* (Qiagen) was added to cultures prior centrifugation (10 min 12,000 g) and total RNA was extracted after lysing bacteria by a 30 min 15 mg.ml-1 lysozyme, 300 U.ml-1 mutanolysin treatment at 20 °C followed by two cycles of Fast-prep (power 6, 30 s) at 4 °C. RNA extraction (Macherey-Nagel RNA extraction kit; Germany) was done according to supplier instructions. RNA integrity was analyzed using an Agilent Bioanalyzer (Agilent Biotechnologies, Ca., USA). 23S and 16S rRNA were depleted from the samples using the MICROBExpress Bacterial mRNA enrichment kit (Invitrogen, France); depletion was controlled on Agilent Bioanalyzer (Agilent Biotechnologies).
description
Exponential phase
developmental stage
emm28
genotype
not collected
geographic location (country and/or sea)
THY
growth condition
not applicable
isolate
Streptococcus pyogenes
organism
Streptococcus pyogenes
organism
Streptococcus pyogenes
scientific_name
WT
strain
WT-THY-Rep3
title