2024-09-20
ENA first public
ERC000050
ENA-CHECKLIST
EMBL Heidelberg
INSDC center name
public
INSDC status
ERS21062397
SRA accession
spire_mag_01580764
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
megahit 1.2.9
assembly software
default
binning parameters
metabat 2.12.1
binning software
human digestive system
broad-scale environmental context
EBI-EMG
broker name
2016-06-09
collection date
90.38
%
completeness score
checkm2 0.1.3
completeness software
1.52
%
contamination score
This sample represents a Third Party Annotation (TPA) binned metagenome assembled from the metagenomic run ERR3342969 of study ERP115515.
description
stool
environmental medium
Ireland
geographic location (country and/or sea)
53.39458800
DD
geographic location (latitude)
-6.35547900
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
human gut metagenome
isolation_source
human gut
local environmental context
human gut metagenome
metagenomic source
Phocaeicola vulgatus
organism
The gut microbiome has begun to be characterised in athlete groups, albeit, to date, only across a subset of sports. This study aimed to determine if the gut microbiome and metabolome differed across sports classification groups among elite Irish athletes, many of whom were participating in the 2016 Summer Olympics. Faecal and urine samples were collected from 37 international level athletes. Faecal samples were prepared for shotgun metagenomic sequencing and faecal and urine samples underwent metabolomic profiling. Dietary intake data was collected using food frequency questionnaires and translated into daily intakes using FETA (Food Frequency Questionnaire European Prospective Investigation into Cancer and Nutrition Tool for Analysis). Differences were observed in the composition and functional capacity of the gut microbiome of athletes across sports groups that were distinguished on the basis of the level of dynamic and static components. The microbiomes of athletes participating in sports with a high dynamic component were found to be most distinct compositionally, while those of athletes participating in sports with high dynamic and static components were the most functionally distinct. Additionally, both microbial (faecal) and human (urine) derived metabolites were found to vary between sports classification groups. In particular cis-aconitate, succinic acid and lactate, in urine samples, and creatinine, in faeces, were found to be significantly different between groups. These differences were evident despite the absence of significant differences in diet. Differences in the gut microbiome and metabolome between groups, in the absence of dietary changes, indicate a role for training load or type as a contributory factor. Further exploration of this hypothesis has the potential to benefit athletes, aspiring athletes and the general public.
project name
SAMEA5665401
sample derived from
Phocaeicola vulgatus
scientific_name
NextSeq 500
sequencing method
multi-marker approach
taxonomic identity marker
binned metagenome: spire_mag_01580764
title