2024-09-20
ENA first public
ERC000050
ENA-CHECKLIST
EMBL Heidelberg
INSDC center name
public
INSDC status
ERS21063307
SRA accession
spire_mag_01883543
Submitter Id
Many fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly quality
megahit 1.2.9
assembly software
default
binning parameters
metabat 2.12.1
binning software
human digestive system
broad-scale environmental context
EBI-EMG
broker name
2011
collection date
95.63
%
completeness score
checkm2 0.1.3
completeness software
0.23
%
contamination score
This sample represents a Third Party Annotation (TPA) binned metagenome assembled from the metagenomic run ERR732916 of study ERP009310.
description
stool
environmental medium
Sweden
geographic location (country and/or sea)
55.70000000
DD
geographic location (latitude)
11.96666667
DD
geographic location (longitude)
metagenome-assembled genome
investigation type
human gut metagenome
isolation_source
human gut
local environmental context
human gut metagenome
metagenomic source
Phocaeicola vulgatus
organism
Bariatric surgery is the most effective procedure for the treatment of obesity. Given the role of the gut microbiota in regulating host metabolism and adiposity, we investigated the long-term effects of bariatric surgery on the human gut metagenome in patients randomized to Roux-en-Y gastric bypass and vertical banded gastroplasty. Genera of the Proteobacteria were specifically enriched after bypass, and the abundance of genes for fatty acid metabolism, nitrogen metabolism and two-component systems was increased after both procedures nine years after surgery. Through colonization of germ-free mice with stools from the patients we demonstrated that the altered gut microbiota promoted reduced fat deposition in recipient mice. Recipients of bypass microbiota also had a lower respiratory quotient, indicating decreased utilization of carbohydrates as fuel. Our results suggest that the gut microbiota may play a direct role in the reduction of adiposity observed after bariatric surgery.
project name
SAMEA3212995
sample derived from
Phocaeicola vulgatus
scientific_name
Illumina HiSeq 2000
sequencing method
multi-marker approach
taxonomic identity marker
binned metagenome: spire_mag_01883543
title