2024-09-20
ENA first public
ERC000011
ENA-CHECKLIST
Division of Stem Cells and Cancer, German Cancer Research Center (DKFZ) and DKFZ-ZMBH Alliance, 69120 Heidelberg, Germany
INSDC center name
public
INSDC status
ERS20949536
SRA accession
E-MTAB-14420:CK397
Submitter Id
79
year
age
ArrayExpress
broker name
mononuclear cell
cell_type
not collected
collection date
human
common name
Protocols: AML samples were collected from diagnostic bone marrow (BM) or peripheral blood (PB) aspirations at the University hospital in Heidelberg in accordance with the Declaration of Helsinki after obtaining written consent from each patient. The project was approved by the Ethics Committee of the Medical Faculty of Heidelberg (NCT-MASTER Platform S-206/2011 and S-169/2017). Mononuclear cells were isolated by density gradient centrifugation using Ficoll Paque Plus (GE Healthcare, cat # GE17-1440-03), and stored in liquid nitrogen until further use. RNA extraction and purification of cells was done using PicoPure RNA Isolation Kit according to manufacturer’s instructions (Thermo Fisher, cat # KIT0214).  Whole transcriptome amplification was performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio, # 634889) according to the manufacturer’s instructions. Tagmentation of cDNA was done using Nextera XT DNA Library Preparation Kit (Illumina, cat # FC-121-1030).
description
acute myeloid leukemia
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
42,X,-X,?3q,del(5)(q31),-7,-17,-18,der(21)t(7;21)(q11.2;q11.2)[27]
karyotype
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
Homo sapiens
scientific_name
female
sex
CK397
title