2024-09-23
ENA first public
ERC000011
ENA-CHECKLIST
Institute of Molecular Genetics of the Czech Academy of Sciences
INSDC center name
public
INSDC status
ERS18822947
SRA accession
E-MTAB-13997:SHPC101
Submitter Id
13.5
age
ArrayExpress
broker name
not collected
collection date
house mouse
common name
Protocols: Molar tooth germs were bilaterally dissected from the mandible at ED 13.5. Tooth primordium was split into anterior and posterior part and posterior part of tooth germs was cultured for 0, 16 or 24 hours respectively on PET track–etched membrane. The samples were harvested in the RNA latter and stored in -20°C. RNA was isolated by standard protocol with Quiagen RNAeasy micro kit and Precellys®24 homogenizator and stored at -80°C. RNA-Seq libraries were generated according to manufacturer’s instructions from 75 ng of total RNA using the Illumina Stranded mRNA Prep, Ligation kit and IDT for Illumina RNA UD Indexes Ligation (Illumina, San Diego, USA)
description
embryo stage
developmental stage
CD1/(B6.CgShhtm1(EGFP/cre)Cjt/J)
genotype
not collected
geographic location (country and/or sea)
E
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
tooth primordium
organism part
Mus musculus
scientific_name
A
sequencing batch
unknown
sex
C57BL/6
strain
SHPC101
title