2024-09-24
ENA first public
ERC000011
ENA-CHECKLIST
Hebrew University
INSDC center name
public
INSDC status
ERS21006564
SRA accession
E-MTAB-14451:Sample 168
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Blister cells were stained for DC subsets and index sorted on FACS ARIA III (BD Biosciences) into 96 well plate containing lysis buffer, one cell/well. Plates were sealed and stored at -80°C. Single-cell cDNA libraries were prepared using the SMARTSeq v2 protocol (Picelli et al., 2014) SMARTSeq v2 protocol (Picelli et al., 2014) with the following modifications: (i) 1 mg/mL BSA Lysis buffer (Ambionâ Thermo Fisher Scientific); and (ii) 200 pg cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA).  SMARTSeq v2 protocol (Picelli et al., 2014) with the following modifications: (i) 1 mg/mL BSA Lysis buffer (Ambionâ Thermo Fisher Scientific); and (ii) 200 pg cDNA with 1/5 reaction of Illumina Nextera XT kit (Illumina, San Diego, CA, USA).
description
not collected
geographic location (country and/or sea)
Blister
injury
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
10
patient
Homo sapiens
scientific_name
Sample 168
title