2024-09-25
ENA first public
ERC000011
ENA-CHECKLIST
Faculty of Biology, Medicine and Health, University of Manchester
INSDC center name
public
INSDC status
ERS20099042
SRA accession
E-MTAB-14093:OE19_RNAseq_rep3
Submitter Id
ArrayExpress
broker name
OE19
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: OE19 cells were grown in DMEM medium containing 10% foetal bovine serum at 37°C incubator with 5% CO2. Cells were transfected using non-target control siRNA for 48 hours, followed by second siRNA transfection. Total RNA was extracted using an RNeasy plus kit (Qiagen, 74134) with DNase treatment according to the manufacturer’s protocol. RNA-seq libraries were constructed following the TruSeq Stranded mRNA sample preparation protocol (Illumina).
description
adult
developmental stage
esophageal adenocarcinoma
disease
ERBB-2amplified
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
esophagus
organism part
replicate 3
replicate
Homo sapiens
scientific_name
OE19_RNAseq_rep3
title