2024-09-25
ENA first public
ERC000011
ENA-CHECKLIST
Unistra
INSDC center name
public
INSDC status
ERS20963145
SRA accession
E-MTAB-14437:TENASCIN_3_PBS-4_BM1_10_N1_T1
Submitter Id
ArrayExpress
broker name
NT193M
cell_line
mesenchymal stem cell of the bone marrow
cell_type
not collected
collection date
house mouse
common name
Protocols: Samples were collected using a cell scraper from cultured cells. NT193 cells were cultured for 18 hours in Dulbecco’s modified Eagle’s medium (DMEM Dutscher L0104-500) containing 4.5 g/L glucose, L-glutamine, sodium pyruvate and supplemented with 10% of heat-inactivated fetal bovine serum (FBS, Dutscher S1810-500), penicillin (100 U/mL), streptomycin (100 μg/mL) (Pan Biotech P06-07100), 2 mM Glutamax (Gibco 35050-061) and Gentamicin sulfate 40 μg/ml (Dutscher L0012- 100). NT193M cells were incubated with MP5 (50 microgr/ml) in 0.1% FCS/DMEM for 72 hours before collection of RNA and RNA seq analysis. The control condition was treated with 1X PBS. Total RNA was extracted using TriReagent (Life Technologies AM9738)  Sequencing libraries were prepared using the Smarter Stranded Total RNA-Seq kit v3 Pico Input Mammalian following the manufacturer’s instructions (Takara Bio, San Jose, CA, USA)
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
mesenchymal stem cell of the bone marrow
organism part
Mus musculus
scientific_name
TENASCIN_3_PBS-4_BM1_10_N1_T1
title