2024-09-25
ENA first public
ERC000011
ENA-CHECKLIST
Faculty of Biology, Medicine and Health, University of Manchester
INSDC center name
public
INSDC status
ERS20104280
SRA accession
E-MTAB-14095:KASseq_dmso_rep3
Submitter Id
ArrayExpress
broker name
OE19
cell_line
epithelial cell
cell_type
not collected
collection date
human
common name
Protocols: OE19 cells were treated with DMSO or 500 nM lapatinib for 1 day or left untreated, and then trypsinised and collected. OE19 cells nucleic acid extraction for KAS-seq was performed as described in Wu T, Lyu R, You Q, He C. Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity in situ. Nat Methods. 2020 17:515–523. Libraries were constructed by amplifying transposed DNA with Illumina Nextera adapters.
description
adult
developmental stage
esophageal adenocarcinoma
disease
ERBB-2amplified
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
esophagus
organism part
Homo sapiens
scientific_name
KASseq_dmso_rep3
title
OE19 treated with DMSO, replicate 3
treatment