Ec191 B4 f
Alternative strain name
140 mm round Petri dish cultures in natural sea water implemented with 0.01% Provasoli enrichment plus antibiotic under 10h:14h light:dark (white fluorescent light of 10-30 mol m-2s-1 photon fluence rate) at 13C. Before harvesting, individuals from each Petri dish were transplanted into freshly prepared culture medium in a new Petri dish and cryotubes for maintenance and further harvesting. Harvesting was carried out as follows: the medium from the Petri dish was discarded. All partheno-sporophytes were collected using a plastic squeegee, rapidly dried on clean absorbent paper, put in a 96 well plate containing a metal ball and frozen in liquid nitrogen. Samples were kept at -80C before DNA extraction. For extraction, tissues were lyophilized and crushed, using the metal ball, in a crusher. Genomic DNA was extracted using the MN Nucleospin Plant DNA kit.
Culture conditions
DNA
DNA or RNA extracted
2024-09-26
ENA first public
ERC000043
ENA-CHECKLIST
01/05/15
Extraction date
NucleoSpin  Plant II, ethanol precipitation
Extraction method
42125
Harvest date
Genoscope CEA
INSDC center name
public
INSDC status
ERS21097946
SRA accession
GPO-29
Submitter Id
Ec846f and Ec847m are sister and brother, inbred for 2 generations. Original designations were B4f and A9m indicating that strains Ec847m  and Ec846f come from two different unilocs on the hterozygous sporophyte Ec191. Ec191 was the product of a cross between Ec188f x Ec189m, which were derived from the same field sporophyte collected at Le Caro, Britanny in 2005. Not known whether Ec188 and Ec189 were derived from the same or different unilocs.
additional details
Akira F. Peters
collected_by
missing: data agreement established pre-2023
collection date
RCC7091
culture_collection
partheno-sporophyte
generation
France
geographic location (country and/or sea)
48.3333
DD
geographic location (latitude)
-4.43333
DD
geographic location (longitude)
Akira F. Peters
identification by
Morphology, genome sequenced
identification method
Ectocarpus fasciculatus
organism
haploid
ploidy
GPO-29
sample alias
DNA extracted from Ectocarpus fasciculatus strain Ec846f for .
sample description
coastal
sampling ecosystem
Atlantic Ocean
sampling sea
Le Caro, Rade de Brest, France
sampling site
marine
sampling water type
Ectocarpus fasciculatus
scientific_name
BSI_CR
sequencing centre sample code
female
sex
genetic cross, genome sequencing
sex determination method
Ec846f
strain
whole thallus, non-fertile
tissue type
DNA extracted from Ectocarpus fasciculatus strain Ec846f.
title