2024-09-29
ENA first public
ERC000011
ENA-CHECKLIST
Department of Medical Biochemistry and Biophysics, Karolinska Institutet
INSDC center name
public
INSDC status
ERS18406378
SRA accession
E-MTAB-13899:QiaseqFX_0_4
Submitter Id
ArrayExpress
broker name
primary cell line
cell_line
fibroblast
cell_type
not collected
collection date
house mouse
common name
Protocols: Cultured mouse tail tip fibroblasts (MTTFs) (CAST/EiJxBL6) were pelleted and stored at -80°C prior to RNA extraction. MTTFs were cultured in standard medium, DMEM with 10% FBS, supplemented with 1x non-essential amino acids and 1x sodium glutamate. Bulk RNA was extracted from cultured mouse tail tip fibroblasts using TRIzol Reagent (Invitrogen). Libraries were constructed using commercially available RNA-seq kits TruSeq RNA Library Preparation Kit v2 (Illumina), NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) and QIAseq FX Single Cell RNA Library Kit (QIAGEN) according to the manufacturers instructions.
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
QIAseqFX
kit
Mus musculus
organism
Mus musculus
organism
tail
organism part
Mus musculus
scientific_name
C57BL/6J x CAST/EiJ
strain
QiaseqFX_0_4
title