2024-09-30
ENA first public
ERC000011
ENA-CHECKLIST
Department of Medicine, Surgery and Dentistry \"Scuola Medica Salernitana\", University of Salerno
INSDC center name
public
INSDC status
ERS18694384
SRA accession
E-MTAB-13990:2h_2
Submitter Id
ArrayExpress
broker name
Mouse macrophages J774A.1  (ATCC, Manassas, VA, USA)
cell_line
macrophage
cell_type
not collected
collection date
house mouse
common name
Protocols: Mouse macrophages J774A.1 cells (ATCC, Manassas, VA, USA) were cultured in RPMI 1640 (Euroclone, Italy), supplemented with 10% (v/v) fetal bovine serum (FBS, Euroclone, South America, origin EU approved), penicillin-streptomycin solution (100 U/mL penicillin and 100 µg/mL streptomycin) (Euroclone, Italy) and grown at 37°C with 5% CO2 in a humidified incubator. Total RNA from J774.1 infected with B. abortus was isolated using Trizol following the manufacturers protocol. The concentration and quality of all isolated RNA samples were measured using the TapeStation 4200 (Agilent Technologies, USA) and Qubit 2.0 fluorometer (Thermo Fisher Scientific, USA). RNA samples that exhibited RNA integrity numbers ranging from 6.6 to 10 were selected for the library preparations. Libraries were prepared using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (Illumina, San Diego, USA) according to the manufacturers instructions, starting with 1 µg total RNA.
description
adult
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
ascites
organism part
Mus musculus
scientific_name
female
sex
BALB/cN
strain
2h_2
title