2024-09-30
ENA first public
ERC000011
ENA-CHECKLIST
Philipps University of Marburg, Center for Synthetic Microbiology
INSDC center name
public
INSDC status
ERS20609121
SRA accession
E-MTAB-11878_2:SmABCΔoriVΔRInv_Nanopore
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Cells were harvested by centrifugation at 3000 x g and 4° C S. meliloti cells were grown in TY supplemented with appropriate antibiotics to an optical density 600 (OD600) of 1.0 (Exp.) or 2.0 (Stat.). Total DNA for resequencing was extracted using a DNeasy Blood and Tissue kit (Qiagen) according to the manual. DNA libraries for sequencing were generated by applying a Nextera XT DNA Library Preparation kit (Illumina) or Ligation Sequencing Kit (Oxford Nanopore).
description
SmABCΔoriV derivative with a 3.2 Mbp genomic inversion
genotype
not collected
geographic location (country and/or sea)
Exponential phase
growth condition
not applicable
isolate
Sinorhizobium meliloti
organism
Sinorhizobium meliloti
organism
Sinorhizobium meliloti
scientific_name
SmABCΔoriVΔRInv_Nanopore
title