2024-09-30
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114469629
External Id
Laboratory of Translational Research, Azienda USL – IRCCS di Reggio Emilia, Reggio Emilia, Italy
INSDC center name
2023-10-08T17:02:25Z
INSDC last update
public
INSDC status
ERS16456253
SRA accession
E-MTAB-13400:linc-0207
Submitter Id
ArrayExpress
broker name
MSTO-211H
cell_line
mesothelial cell
cell_type
not collected
collection date
human
common name
Protocols: Cells were collected after 72 hours from transfection. MSTO-211H were cultured in RPMI GlutaMax medium added with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. Cells were grown at 37°C, 5% CO2. cells were reverse-transfected with siRNA against linc00941 or Negative Control siRNA at a final concentration of 20 nM. Total RNA was purified with Maxwell® RSC simplyRNA Cells (Promega). RNAseq libraries were prepared by Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit starting from 500 ng of RNA. RNA quality was assessed by Bioanalyzer RNA 6000 nano kit.
description
adult
developmental stage
malignant pleural mesothelioma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
lung
organism part
1
replicate
Homo sapiens
scientific_name
linc-0207
title