SAMN37864232
External Id
Oregon State University
INSDC center name
2024-10-01T00:53:47.400Z
INSDC first public
2024-10-01T00:53:47.400Z
INSDC last update
SRS19190258
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.microbial
NCBI submission model
MIMARKS.survey
NCBI submission model
MIMARKS.survey.microbial.6.0
NCBI submission package
SRS19190258
SRA accession
2016-07-20
collection_date
0 cm
depth
USGS_1153550_WILLIAMS RIVER NEAR ROCKINGHAM, VT
description
not collected
elev
temperate watershed
env_broad_scale
river
env_local_scale
epilithon
env_medium
USA:Vermont
geo_loc_name
43.1917433 N 72.48508889 W
lat_lon
freshwater metagenome
http://purl.obolibrary.org/obo/NCBITaxon_449393
organism
To sample biofilm communities, eight rocks were randomly selected and scrubbed with a sterile brush for 30 seconds within the area of a 25cm^3 circular polypropylene stencil in a sterile polypropylene bin. Loosened biofilm material from 8 rocks was suspended in sterile site water pre-filtered through 0.22 µm Sterivex filters, pooled, transferred to a sterile 60 mL syringe, and filtered through a 0.22 µm Sterivex filter until the filter clogged.
samp_collect_device
Sample collected on a 0.2 micron pore-size Millipore Sterivex-GP filter capsule with a peristaltic pump. DNA extracted with phenol-chloroform following Crump et al. (2013) DOI: 10.1128/AEM.69.4.2253-2268.2085
samp_mat_process
PCA_167_WILL_20-Jul-2016_DNA_biofilm
title