SAMN37864267
External Id
Oregon State University
INSDC center name
2024-10-01T00:50:46.310Z
INSDC first public
2024-10-01T00:50:46.310Z
INSDC last update
SRS19190297
INSDC secondary accession
live
INSDC status
MIGS/MIMS/MIMARKS.microbial
NCBI submission model
MIMARKS.survey
NCBI submission model
MIMARKS.survey.microbial.6.0
NCBI submission package
SRS19190297
SRA accession
2016-04-21
collection_date
0 cm
depth
USGS_1188090_FARMINGTON RIVER AT UNIONVILLE, CT
description
not collected
elev
temperate watershed
env_broad_scale
river
env_local_scale
epilithon
env_medium
USA:Connecticut
geo_loc_name
41.7555472 N 72.8870417 W
lat_lon
freshwater metagenome
http://purl.obolibrary.org/obo/NCBITaxon_449393
organism
To sample biofilm communities, eight rocks were randomly selected and scrubbed with a sterile brush for 30 seconds within the area of a 25cm^3 circular polypropylene stencil in a sterile polypropylene bin. Loosened biofilm material from 8 rocks was suspended in sterile site water pre-filtered through 0.22 µm Sterivex filters, pooled, transferred to a sterile 60 mL syringe, and filtered through a 0.22 µm Sterivex filter until the filter clogged.
samp_collect_device
Sample collected on a 0.2 micron pore-size Millipore Sterivex-GP filter capsule with a peristaltic pump. DNA extracted with phenol-chloroform following Crump et al. (2013) DOI: 10.1128/AEM.69.4.2253-2268.2120
samp_mat_process
PCA_258_UNIO_21-Apr-2016_DNA_biofilm
title