2024-10-03
ENA first public
ERC000011
ENA-CHECKLIST
Génomes/I2BC/UMR9198-CEA/CNRS/Université Paris-Saclay
INSDC center name
public
INSDC status
ERS20302679
SRA accession
E-MTAB-14201:S17_Abdomen_40Gy_Rep2
Submitter Id
ArrayExpress
broker name
primary cell line
cell_line
fibroblast
cell_type
not collected
collection date
human
common name
Protocols: Cell monolayers were trypsinized, neutralized with medium, washed with PBS, and centrifuged. Washed cell pellets were resuspended in Macherey-Nagel Nucleospin RNA Plus Lysis Buffer. No dissections were performed so this protocol is not applicable. MEM + 10%FBS + sodium pyruvate + non-essential amino acids + Gluta-Max + pen/strep in 5% 02 + 5% CO2 20 or 40 Gy X-rays at 120 kV generated by a Faxitron 43855-D generator The following software were used for data conversion: EdgeR Limma voomrmalization and data  RNA was extracted with the Macherey-Nagel Nucleospin RNA Plus. NEBNext Ultra II Directional RNA Library Kit with PolyA RNA selection
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
fibroblast
organism part
Homo sapiens
scientific_name
40 Gy X-ray 120kV
stimulus
S17_Abdomen_40Gy_Rep2
title