2024-10-03
ENA first public
ERC000011
ENA-CHECKLIST
College of Biomedical Engineering, Taipei Medical University, Taiwan
INSDC center name
public
INSDC status
ERS18631898
SRA accession
E-MTAB-13966:EV23
Submitter Id
ArrayExpress
broker name
cardiomyocyte
cell_type
not collected
collection date
human
common name
Protocols: RNA-seq was performed on cultured human induced pluripotent cell derived cardiomyocytes (iPSC-CMs).  There are four groups, with three samples per group: H1,H2,H3. Negative control. Healthy, normoxic iPSC-CMs D1,D2,D3. Positive control. iPSCs cultured under hypoxic (0-1% O2) for 48h with 2% v/v PBS as vehicle control EV1,EV2,EV3. Treatment 1. iPSCs cultured under hypoxic (0-1% O2) for 48h, with cardiac stromal cell derived extracellular vesicles, provided at a dose of 67ng/µl (2% v/v) EV21, EV23, EV24. Treatment 2 iPSCs cultured under hypoxic (0-1% O2) for 48h, with bone marrow mesenchymal stromal cell (BM-MSC) derived extracellular vesicles, provided at a dose of 67ng/µl (2% v/v)  All EVs were isolated from cultured human cells using sequential centrifugation methods.  Cells were cultured using commercial EV-depleted FBS to avoid contamination of bovine EVs.   EVs were validated for CD81, CD9, ALIX positivity, and visualised by cryoEM.  Particle to protein ratios were not different between cardiac and bone marrow EV isolates.  Therefore EV doses were standardised so that the same dose by protein (67ng/µl) and EV number (~2,000 EVs per iPSC-CM) were added. RNA was extracted from hiPSC-CMs using a kit (Qiagen RNAeasy Micro, 217084) Agilent SureSelect XT HS2 mRNA Library Prep Kit
description
adult
developmental stage
normal
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
Hypoxia
growth condition
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
induced pluripotent stem cell
progenitor cell type
Homo sapiens
scientific_name
bone marrow mesenchymal stromal cell derived extracellular vesicles
stimulus
EV23
title