2024-10-04
ENA first public
ERC000011
ENA-CHECKLIST
Laboratory of Molecular Medicine  and Genomics
INSDC center name
public
INSDC status
ERS18400105
SRA accession
E-MTAB-13885:Sample 2
Submitter Id
ArrayExpress
broker name
MCF7
cell_line
breast invasive ductal carcinoma cell
cell_type
not collected
collection date
human
common name
Protocols: MCF-7 were purchased from the American Type Culture Collection (ATCC). Cell lines were cultured according to manufacturer’s protocol and as indicated,  MCF-7 cell lines treated with 10 µM GSK6853 and its vehicle for 6 days. Biological replicates were carried out in duplicate and ~100,000 nuclei were loaded per reaction  collecting directly into the cold ATAC-seq lysis buffer (Active Motif cat. 53150). Library preparation was carried out per manufacturer’s instructions (Active Motif cat. 53150). Indexed library concentrations were measured using Quant-IT RNA Assay Kit-High Sensitivity and a Qubit Fluorometer (Life Technologies, Monza, Italy) and integrity assessed with the Agilent 4200 Tapestation System (Agilent Technologies, Milan, Italy)
description
breast cancer
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
breast
organism part
Homo sapiens
scientific_name
Sample 2
title