2024-10-05
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA111436323
External Id
Laboratory of Traslational Research. Azienda USL-IRCCS di Reggio Emilia
INSDC center name
2022-10-05T10:43:39Z
INSDC last update
public
INSDC status
ERS13529998
SRA accession
E-MTAB-12244:MET113_INPUT
Submitter Id
79
year
age
ArrayExpress
broker name
human
common name
Protocols: Fresh frozen tissue samples were provided by the biobank of Azienda USL-IRCCS of Reggio Emilia. Tissues were homogenized with dounce homogenizer in 1% PBS, then crosslinked for 15 minutes with 1% formaldehyde, lysed and sonicated for 12 cycles (30” ON, 30” OFF) using Bioruptor Pico Sonicator. Chromatin was precipitated overnight using Dynabeads Protein G magnetic beads and 3 µg of anti H3K27ac (Abcam). 1% of total chromatin was used as input. Library was prepared following NEBNext Ultra II DNA Library Prep Kit for Illumina using 5-20 ng ChIP DNA as starting material.
description
adult
developmental stage
melanoma
disease
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
female
sex
MET113_INPUT
title
metastasis
tumor grading