2024-10-08
ENA first public
ERC000011
ENA-CHECKLIST
INSERM Gustave Roussy
INSDC center name
public
INSDC status
ERS20174307
SRA accession
E-MTAB-14140:Foetal_Liver_EG_NSG_S
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Hematopoietic cells expressing the ETO2-GLIS2 fusion were purified by flow cytometry including for cell viability (using Sytox blue-negative cells). Sample preparation was done at room temperature. Single-cell suspensions were loaded onto a Chromium Single Cell Chip (10x Genomics) according to the manufacturer’s instructions for co-encapsulation with barcoded Gel Beads at a target capture rate of ~10,000 individual cells per sample. Captured mRNAs were barcoded during cDNA synthesis using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10X Genomics) according to the manufacturer’s instructions. All samples were processed simultaneously with the Chromium Controller (10X Genomics) and the resulting libraries were prepared in parallel in a single batch.
description
fetus
developmental stage
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
bone marrow
organism part
EG_NSG_S
sample group
Homo sapiens
scientific_name
Foetal_Liver_EG_NSG_S
title