2024-10-10
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA114761011
External Id
Sahlgrenska Center for Cancer Research Department of Microbiology and Immunology Institute of Biomedicine Sahlgrenska Academy University of Gothenburg
INSDC center name
2023-11-28T13:15:26Z
INSDC last update
public
INSDC status
ERS17180699
SRA accession
E-MTAB-13567_2:Sample 2
Submitter Id
11
week
age
ArrayExpress
broker name
MLL-AF9 leukemia cell
cell_type
not collected
collection date
house mouse
common name
Protocols: 100,000 bone marrow cells from terminally sick leukemia-engrafted mice were transplanted into sublethally irradiated (475 rad) recipient mice. Bone marrow cells were harvested from leukemia-engrafted mice that were administered Dox for six days and viable GFP+ leukemia cells were sorted into PBS containing 0.05 % BSA following the manusfacturer’s protocol. For each sample, 15,000 cells were loaded in a 10x Genomics Chromium Next GEM Chip G channel. Libraries were constructed using 10x Genomics Chromium Next GEM Single Cell 3' v3.1 kit and the quality of sequencing libraries was determined using Agilent 4200 Tapestation system according to manufacturer’s instructions.
description
adult
developmental stage
normal
diet
[M2-rtTA/M2-rtTA][EIF6/EIF6]
genotype
not collected
geographic location (country and/or sea)
NoDox_2
individual
Mus musculus
organism
Mus musculus
organism
bone marrow
organism part
Mus musculus
scientific_name
female
sex
C57BL/6
strain
Sample 2
title