2024-10-10
ENA first public
ERC000011
ENA-CHECKLIST
Universidad de León
INSDC center name
public
INSDC status
ERS18822713
SRA accession
E-MTAB-13994:Sample 9
Submitter Id
25
month
age
ArrayExpress
broker name
not collected
collection date
sheep
common name
Protocols: Ten first lactation Assaf ewes were used in this study. Individual milk samples from both mammary glands of each animal were collected at the three time points studied (0h, 6h and 24h) to measure the somatic cell count (SCC) and evaluate the response to the inoculated LPS. Briefly, from each ewe, 50 ml of milk from the inoculated half of the mammary gland was used for RNA extraction. To avoid RNA degradation previously sampling collection, the udder was disinfected with povidone iodine, and nipples were cleaned with RNAseZap (Ambion, Austin, TX, USA). A sterile gauze was used to cover the 50 ml RNAse-free tubes during collection. For RNA extraction, MSCs were pelleted by centrifugation at 650×g for 10 minutes at 4 °C and in the presence of a final concentration of 0.5 mM of EDTA. Then, the MSC pellet was washed twice with 10, 2 mL of PBS (pH 7.2 and 0.5 mM of EDTA) followed by centrifugation at 650×g for 10 min at 4°C. The RNA extraction was performed using 500 μL of TRIzol according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA).  Paired-end libraries were prepared using the UltraTM RNA Library Prep Kit (NEBNext®).
description
primiparous
developmental stage
E coli LPS inoculation
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
C_P24h_1
individual
not applicable
isolate
Ovis aries
organism
Ovis aries
organism
mammary gland
organism part
Ovis aries
scientific_name
female
sex
Assaf
strain
Sample 9
title