2024-10-13
ENA first public
ERC000011
ENA-CHECKLIST
Genetics and Genomics of Insect Vectors Unit, Department of Parasites and Insect Vectors, Institut Pasteur, Université de Paris Cité, CNRS UMR2000, F-75015 Paris, France
INSDC center name
public
INSDC status
ERS21097505
SRA accession
E-MTAB-14478:dsENH07_3
Submitter Id
3
day
age
ArrayExpress
broker name
not collected
collection date
African malaria mosquito
common name
Protocols: Three-day old female females Anopheles coluzzii mosquitoes were injected with dsENH07 or dsGFP, and after 2 days, total RNAs from three biological replicates were extracted and sequenced. For each biological replicate, pools of ~10 mosquitoes were extracted per treatment group. Three-day-old cold-anaesthetized mosquito females were injected using the NanoJect II (Drummond) either with 1.5mg of an equal quantity of ds1, ds2 and ds3 (500 ng each per mosquito) or with 1.5mg of dsGFP for the control group as described above. Two days post-injection, for each condition, total RNA was extracted from a pool of 10 mosquitoes with TRI Reagent (Molecular Research Center, Inc.). The use of 3dsRNA was to optimize the targeting and silencing of the ENH07 eRNA. Three independent experiments were performed and the extracted total RNA from each condition and each experiment were submitted to transcriptome analysis.  total RNA was extracted from a pool of 10 mosquitoes with TRI Reagent (Molecular Research Center, Inc.) The libraries were prepared with the illumina TruSeq Stranded mRNA kit
description
adult
developmental stage
ENH07-KD
genotype
not collected
geographic location (country and/or sea)
Pool of 10 mosquitoes
individual
not applicable
isolate
Anopheles gambiae
organism
Anopheles gambiae
organism
whole body
organism part
Anopheles gambiae
scientific_name
female
sex
M
strain
dsENH07_3
title