2024-10-14
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA111476235
External Id
CENTRO RICERCA TETTAMANTI, UNIV. MILANO-BICOCCA, MONZA, ITALY
INSDC center name
2022-10-14T15:37:16Z
INSDC last update
public
INSDC status
ERS13570271
SRA accession
E-MTAB-12300:Sample 15 KW5
Submitter Id
8
week
age
ArrayExpress
broker name
house mouse
common name
Protocols: OCT colon section. QIAGEN RNAeasy mini kit following the standard protocol according to the manufacturer \"Purification of total RNA from animal tissues\". Whole transcriptome RNA-seq analyses have been performed by Next Generation Sequencing (NGS), using the Universal RNAseq kit (Nugen, Tecan). The Universal RNA-Seq is an end-to-end solution for strand-specific RNA-Seq library construction used 250 ng nanogram amounts of total RNA obtained from gut sections. The workflow consists of double-stranded cDNA generation using a mixture of random priming, optional fragmentation of double-stranded cDNA, end repair, adaptor ligation, strand selection, targeted transcript depletion with AnyDeplete for globin genes, and PCR amplification to produce the final library. The yields of final libraries were assessed by Qubit 4.0 fluorimeter and their sizing by Agilent Bioanalyzer.
description
adult
developmental stage
graft versus host disease
disease
wild type genotype
genotype
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
large intestine
organism part
Mus musculus
scientific_name
male
sex
BALB/c
strain
Sample 15 KW5
title
allotransplantation with C57BL/6 wild type monocytes and CMKLR1 -/- donor cells
xenograft