2024-10-14
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA111468648
External Id
UNIVERSITY OF MILANO-BICOCCA
INSDC center name
2022-10-14T09:23:56Z
INSDC last update
public
INSDC status
ERS13562678
SRA accession
E-MTAB-12278:Sample 12_B6_TUM
Submitter Id
14
week
age
ArrayExpress
broker name
neutrophil
cell_type
house mouse
common name
Protocols: AOM-DSS model: 7 weeks old mice were injected intraperitoneally with 10 mg/kg body weight Azoxymethane (AOM, Merck), dissolved in isotonic saline solution. After 7 days, mice were given 1% (w/v) dextran sodium sulfate (DSS MW 40 kD; TdB Consultancy) in their drinking water for 7 days followed by 14 days of recovery. The cycles were repeated 2 times and mice sacrificed at day 49. Neutrophils were sorted from tumoral lesions or healthy colon of 14 weeks/old female TRAJ18-/- [B6(Cg)-Traj18tm1.1Kro/J; JAX stock: 030524; RRID:IMSR_JAX:030524] and C57BL/6J [The Jackson Laboratory JAX stock: 000664; RRID:IMSR_JAX:000664] mice. 5 × 105 neutrophils (CD45+CD11b+Ly6G+) were sorted with a BD FACSaria and resuspended in RNAlater before storage at -80.c Total RNA from ~ 5 × 105 neutrophils (CD45+CD11b+Ly6G+) was isolated with the RNeasy micro kit (Qiagen) and RNA quality was checked with the Agilent 2100 Bioanalyzer Sequencing libraries were prepared by using the NEBNext® rRNA Depletion Kit v2 and the NEBNext® Ultra™ II Directional RNA Library Prep kits following manufacturer’s instructions.
description
colorectal cancer
disease
wild type genotype
genotype
CD45+CD11b+Ly6G+
immunophenotype
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
colon
organism part
Mus musculus
scientific_name
female
sex
C57BL/6
strain
Sample 12_B6_TUM
title