2024-10-15
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Discovery Brain Sciences, University of Edinburgh
INSDC center name
public
INSDC status
ERS20536010
SRA accession
E-MTAB-14233:G_MP
Submitter Id
10 to 12
month
age
ArrayExpress
broker name
astrocyte
cell_type
not collected
collection date
house mouse
common name
Protocols: To sort astrocytes, mouse neocortices were rapidly harvested then dissociated with Adult Brain Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator using program 37C_ABDK_01. Dissociated samples were then treated with debris and red blood cell removal steps to obtain cell suspensions. For FACS, cells were resuspended in a final volume of 100 uL 0.1% PB buffer. Then cells were incubated with fluorescent conjugated antibodies at 4° C for 30 minutes. The following antibodies were used: ACSA2 APC (1:200, Miltenyi Biotec, 130-116-245) and O4 PE (1:100, Miltenyi Biotec, 30-117-357). ACSA2 APC (+) and O4-PE (-) live single cells were selected as the astrocyte population.   Library preparation was conducted with the Chromium Single Cell 3′ v3.1 Reagent Kit (10x Genomics), following the manufacturer’s instructions. Library preparation was conducted with the Chromium Single Cell 3′ v3.1 Reagent Kit (10x Genomics), following the manufacturer’s instructions.
description
adult
developmental stage
HET_APP-HOM_FIRE
genotype
not collected
geographic location (country and/or sea)
430
individual
not applicable
isolate
Multiplexing
library type
CMO309
multiplexing cmos
Mus musculus
organism
Mus musculus
organism
cerebral cortex
organism part
Mus musculus
scientific_name
male
sex
CBA
strain
G_MP
title