2024-10-15
ENA first public
ERC000011
ENA-CHECKLIST
DKFZ
INSDC center name
public
INSDC status
ERS18989384
SRA accession
E-MTAB-13700:XXT-02
Submitter Id
12
week
age
ArrayExpress
broker name
all liver types
cell_type
not collected
collection date
house mouse
common name
Protocols: Livers were dissected and flash frozen in liquid nitrogen then subsequently stored at -80 degrees C.  Frozen livers were pulverized with a cryoprep, and a portion of frozen liver powder was lysed in a hypotonic PBS-based buffer with Igepal. During cell lysis, samples were dounced to liberate nuclei and subsequently fixed with 85% MeOH and DTSSP.  Fixed nuclei were distributed across wells of a 96-well plate, with individual mice indexed by RT well, and subjected to sci-RNA-seq3.
description
adult
developmental stage
Chr3-Sry+
genotype
not collected
geographic location (country and/or sea)
1315598
individual
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
liver
organism part
Mus musculus
scientific_name
male
sex
C57BL6/J
strain
XXT-02
title