2024-10-16
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA112753323
External Id
Institute of Molecular Oncology, Philipps-University Marburg
INSDC center name
2023-03-07T16:19:40Z
INSDC last update
public
INSDC status
ERS14748013
SRA accession
E-MTAB-12734:R249S_DMSO_1
Submitter Id
ArrayExpress
broker name
HCT116
cell_line
human
common name
Protocols: Cells were detached using 0.05 % Trypsin-EDTA (Gibco, 15400054). The human colorectal carcinoma cell line HCT116 were obtained from the American Tissue Collection Center (ATCC, CCL-247) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, 41966-029) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Sigma-Aldrich, S0615) and 100 U/ml penicillin and 100 µg/ml streptomycin (Gibco, 15140122). Cell lines were cultivated in a humidified atmosphere at 37 °C and 5% CO2. Cells were treated either with 10 µM N3a or the corresponding volume of DMSO, as solvent control, for 36 h prior to RNA isolation. RNA isolation was done using the RNeasy Mini Kit according to manufacturer’s protocol. RNA quality was evaluated using the Experion RNA StdSens Analysis Kit (Bio‐Rad, 700-7103). RNAseq libraries were prepared from total RNA with the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, 015.24) in combination with the UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) (Lexogen, 081.96) following to manufacturer's protocol.
description
adult
developmental stage
colorectal cancer
disease
TP53 Δ/R249S
genotype
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
colon
organism part
Homo sapiens
scientific_name
R249S_DMSO_1
title