2024-10-18
ENA first public
ERC000011
ENA-CHECKLIST
Universitätsklinikum Erlangen Division of Molecular and Experimental Surgery
INSDC center name
public
INSDC status
ERS20394975
SRA accession
E-MTAB-14208:ERN91_238089
Submitter Id
ArrayExpress
broker name
primary cell line
cell_line
Normal associated fibroblast
cell_type
not collected
collection date
human
common name
Protocols: 6 human CAF lines from human CRC and 3 matching NAFs from uninvolved healthy colon were isolated as described in Naschberger et al, JoVE2018 and Schellerer et al, Oncol Rep 2014 and propagated in culture for up to two passages prior to RNA extraction. Fibroblasts were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, #S0615) 1% L-Glutamin (Thermo Fisher, #25030-024) and 1% Penicillin/Streptomycin (Gibco, #15140-122) at 37°C, with 8.5% CO2 and 95% humidity. RNA was extracted from cells using the RNeasy Mini Kit (Qiagen) following the manufacturers instruction. For each sample, a cDNA library was constructed using the TruSeq Stranded mRNA Kit (Illumina).
description
adult
developmental stage
colorectal cancer
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
colorectum
organism part
Homo sapiens
scientific_name
female
sex
ERN91_238089
title