ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
ERS16590014
SRA accession
EMOBON00010
Submitter Id
10
L
amount or size of sample collected
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023]
broad-scale environmental context
2021-07-01
collection date
42401.005
mS/cm
conductivity
CTD
conductivity method
1.024036
g/m3
density
CTD
density method
20.54
m
depth
EMOBON metagenome water sample from station BPNS collected on 2021-07-01 size fraction 0.2-3um
description
7.49
µmol/kg
dissolved oxygen
CTD
dissolved oxygen method
19.2
ng/µl
dna concentration
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
environmental medium
Belgium
geographic location (country and/or sea)
51.433331
DD
geographic location (latitude)
Belgian part of the North Sea
geographic location (locality)
2.808331
DD
geographic location (longitude)
North Atlantic Ocean
geographic location (ocean)
North Sea
geographic location (region and locality)
59885936.0
library reads sequenced
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
local environmental context
mock_eu
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
mock_pr
3.03
µmol/L
nitrate
SEAL QuAAtro analysis system + Skalar AutoAnalyser system
nitrate method
0.2
µmol/L
nitrite
SEAL QuAAtro analysis system + Skalar AutoAnalyser system
nitrite method
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
BPNS
obs_id
marine plankton metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
0.99965457685
atm
pressure
CTD
pressure method
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
220712_JARVIS_HMNJKDSX3
run
33.088
psu
salinity
CTD
salinity method
Niskin Bottle
sample collection device
WaSOP1
sample collection method
WaSOP1
sample material processing
MSO LifeWatch Lab -80 Freezer
sample storage location
-80
°C
sample storage temperature
marine plankton metagenome
scientific_name
16.817
ºC
sea subsurface temperature
CTD
sea subsurface temperature method
16.848
ºC
sea surface temperature
CTD
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220712
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
-0.53
µmol/L
silicate
SEAL QuAAtro analysis system + Skalar AutoAnalyser system
silicate method
0.2-3
μm
size fraction
0.2
µm
size-fraction lower threshold
3
µm
size-fraction upper threshold
EMOBON_BPNS_Wa_210701_0.2um_1
source material identifiers
sample
status
32.979
psu
subsurface salinity
CTD
subsurface salinity method
metag
target gene
metag
target subfragment
16.817
ºC
temperature
low
tidal stage
ebb_tide
tidal stage information
EMOBON_BPNS_Wa_7
title
26
m
total depth of water column