ERC000021
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
ERS15538559
SRA accession
EMOBON00087
Submitter Id
10
L
amount or size of sample collected
marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
broad-scale environmental context
2021-08-25
collection date
20.54
m
depth
EMO BON marine sediment metagenome sample from station BPNS, collected 2021-08-25, micro.
description
7.19
µmol/kg
dissolved oxygen
CTD
dissolved oxygen method
5.56
dna concentration
-20.54
m
elevation
sediment [ENVO:00002007]
environmental medium
Belgium
geographic location (country and/or sea)
51.433331
DD
geographic location (latitude)
Belgian part of the North Sea
geographic location (locality)
2.80833
DD
geographic location (longitude)
North Atlantic Ocean
geographic location (ocean)
North Sea
geographic location (region and locality)
52460513
library reads sequenced
shallow_marine_sediment_[ENVO:03000034]
local environmental context
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
nucleic acid extraction
BPNS
obs_id
marine sediment metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
1.011004194
atm
pressure
CTD
pressure method
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4
relevant standard operating procedures
220712_JARVIS_HMNJKDSX3
run
33.348
practical salinity unit
salinity
CTD
salinity method
Van Veen Grab
sample collection device
SoSOP3
sample material processing
MSO LifeWatch Lab -80 Freezer
sample storage location
-80
°C
sample storage temperature
0.75
g
sample volume or weight for DNA extraction
marine sediment metagenome
scientific_name
18.919
ºC
sea surface temperature
CTD
sea surface temperature method
18.814
ºC
sediment temperature
CTD
sediment temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220712
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
EMOBON_BPNS_So_210825_micro_3
source material identifiers
sample
status
metag
target gene
metag
target subfragment
low
tidal stage
ebb_tide
tidal stage information
EMOBON_BPNS_So_13
title
26
m
total depth of water column