ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
ERS16590017
SRA accession
EMOBON00013
Submitter Id
7
L
amount or size of sample collected
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023]
broad-scale environmental context
7.009
µg/L
chlorophyll
HPLC
chlorophyll method
2021-08-25
collection date
444968.308
mS/cm
conductivity
CTD
conductivity method
1.023729
g/m3
density
CTD
density method
18.66
m
depth
EMOBON metagenome water sample from station BPNS collected on 2021-08-25 size fraction 0.2-3um
description
7.19
µmol/kg
dissolved oxygen
CTD
dissolved oxygen method
34.4
ng/µl
dna concentration
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
environmental medium
Belgium
geographic location (country and/or sea)
51.433331
DD
geographic location (latitude)
Belgian part of the North Sea
geographic location (locality)
2.808331
DD
geographic location (longitude)
North Atlantic Ocean
geographic location (ocean)
North Sea
geographic location (region and locality)
50474845.0
library reads sequenced
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
local environmental context
mock_eu
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
mock_pr
1.75
µmol/L
nitrate
SEAL QuAAtro analysis system + Skalar AutoAnalyser system
nitrate method
1.95
µmol/L
nitrite
SEAL QuAAtro analysis system + Skalar AutoAnalyser system
nitrite method
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
BPNS
obs_id
marine plankton metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
1.01100419442
atm
pressure
CTD
pressure method
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
220712_JARVIS_HMNJKDSX3
run
33.348
psu
salinity
CTD
salinity method
Niskin Bottle
sample collection device
WaSOP1
sample collection method
WaSOP1
sample material processing
MSO LifeWatch Lab -80 Freezer
sample storage location
-80
°C
sample storage temperature
marine plankton metagenome
scientific_name
18.919
ºC
sea subsurface temperature
CTD
sea subsurface temperature method
18.919
ºC
sea surface temperature
CTD
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220712
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
1.63
µmol/L
silicate
SEAL QuAAtro analysis system + Skalar AutoAnalyser system
silicate method
0.2-3
μm
size fraction
0.2
µm
size-fraction lower threshold
3
µm
size-fraction upper threshold
EMOBON_BPNS_Wa_210825_0.2um_1
source material identifiers
sample
status
33.45
psu
subsurface salinity
CTD
subsurface salinity method
metag
target gene
metag
target subfragment
18.919
ºC
temperature
low
tidal stage
ebb_tide
tidal stage information
EMOBON_BPNS_Wa_26
title
26
m
total depth of water column