2024-10-24
ENA first public
ERC000021
ENA-CHECKLIST
University of Bremen
INSDC center name
public
INSDC status
ERS21055640
SRA accession
190283:f8340e7a-c5b3-43ec-a2f7-ef13ba251630
Submitter Id
0.002
L
amount or size of sample collected
marine sediment [ENVO:03000033] | coast [ENVO:01000687]
broad-scale environmental context
GFBIO
broker name
2017-04-19T00:00
collection date
2021-04-10T00:00
collection date experiment
45-70
m
depth
Slurry incubation experiment with antibiotics and sulfate, timepoint day 98, incubated at 20
            degrees C, sediment from station 2-2, coreHE483, Helgoland mud area, North Sea, Germany - Archaeal 16S rRNA
            gene community (DNA)
description
-28.8
m
elevation
anaerobic sediment [ENVO:00002045] | mud [ENVO:01000001]
environmental medium
sediment
environmental package
time series design [EFO:0001779] | different substrate additions
experimental factor
North Sea
geographic location (country and/or sea)
54.087627
DD
geographic location (latitude)
7.968191
DD
geographic location (longitude)
Helgoland mud area, North Sea, Germany
geographic location (region and locality)
mimarks-survey
investigation type
microbial community [PCO:1000004] | mesocosm [ENVO:01000620]
local environmental context
253
nominal_length_noprim
marine sediment metagenome
organism
anaerobic
oxygenation status of sample
initial denaturation:95_3; denaturation:98_0.2;annealing:60_0.15;elongation:72_0.15;final
                    elongation:72_1
pcr conditions
FWD:CAGCMGCCGCGGTAA;REV:GGACTACVSGGGTATCTAAT
pcr primers
Characterization of uncultivated Thermoplasmatota in anaerobic marine sediments of the Helgoland
                    mud area
project name
fwd-rev
read_direction
anaerobe
relationship to oxygen
A
replicate experiment
syringe, needle
sample collection device
slurry sampling in anoxic manner
sample collection method
slurry sample was frozen at -20 degrees C until DNA extraction
sample material processing
2
mL
sample volume or weight for DNA extraction
Slurry incubation experiment with antibiotics and sulfate, timepoint day 98, incubated at 20
                    degrees C, sediment from station 2-2, coreHE483, Helgoland mud area, North Sea, Germany - Archaeal
                    16S rRNA gene community (DNA)
sample_description
Helgoland mud area slurry incubation - antibiotics.sulfate - day 98
sample_title.1
marine sediment metagenome
scientific_name
sediment
sediment environmental package
HL32NDRXY_L1
sequencelane_id
Illumina NovaSeq 6000
sequencing method
slurry prepared in ratio 1:4 of sediment Helgoland mud area core 583/2-2 and sulfate-free
                    artificial sea water; 5mL slurry transferred into anaerobic Widdel medium after 372 days
source material identifiers
true
submitted to insdc
antibiotics, sulfate
substrated added experiment
V4
target subfragment
Helgoland mud area slurry incubation - antibiotics.sulfate - day 98
title
28.8
m
total depth of water column