2024-10-24
ENA first public
ERC000021
ENA-CHECKLIST
University of Bremen
INSDC center name
public
INSDC status
ERS21055638
SRA accession
190285:f8340e7a-c5b3-43ec-a2f7-ef13ba251630
Submitter Id
0.002
L
amount or size of sample collected
marine sediment [ENVO:03000033] | coast [ENVO:01000687]
broad-scale environmental context
GFBIO
broker name
2017-04-19T00:00
collection date
2021-04-10T00:00
collection date experiment
45-70
m
depth
Slurry incubation experiment with protein,antibiotics and sulfate, timepoint day 98, incubated at
            20 degrees C, sediment from station 2-2, coreHE483, Helgoland mud area, North Sea, Germany - Archaeal 16S
            rRNA gene community (DNA)
description
-28.8
m
elevation
anaerobic sediment [ENVO:00002045] | mud [ENVO:01000001]
environmental medium
sediment
environmental package
time series design [EFO:0001779] | different substrate additions
experimental factor
North Sea
geographic location (country and/or sea)
54.087627
DD
geographic location (latitude)
7.968191
DD
geographic location (longitude)
Helgoland mud area, North Sea, Germany
geographic location (region and locality)
mimarks-survey
investigation type
microbial community [PCO:1000004] | mesocosm [ENVO:01000620]
local environmental context
253
nominal_length_noprim
marine sediment metagenome
organism
anaerobic
oxygenation status of sample
initial denaturation:95_3; denaturation:98_0.2;annealing:60_0.15;elongation:72_0.15;final
                    elongation:72_1
pcr conditions
FWD:CAGCMGCCGCGGTAA;REV:GGACTACVSGGGTATCTAAT
pcr primers
Characterization of uncultivated Thermoplasmatota in anaerobic marine sediments of the Helgoland
                    mud area
project name
fwd-rev
read_direction
anaerobe
relationship to oxygen
A
replicate experiment
syringe, needle
sample collection device
slurry sampling in anoxic manner
sample collection method
slurry sample was frozen at -20 degrees C until DNA extraction
sample material processing
2
mL
sample volume or weight for DNA extraction
Slurry incubation experiment with protein,antibiotics and sulfate, timepoint day 98, incubated at
                    20 degrees C, sediment from station 2-2, coreHE483, Helgoland mud area, North Sea, Germany -
                    Archaeal 16S rRNA gene community (DNA)
sample_description
Helgoland mud area slurry incubation - protein.antibiotics.sulfate - day 98
sample_title.1
marine sediment metagenome
scientific_name
sediment
sediment environmental package
HL32NDRXY_L1
sequencelane_id
Illumina NovaSeq 6000
sequencing method
slurry prepared in ratio 1:4 of sediment Helgoland mud area core 583/2-2 and sulfate-free
                    artificial sea water; 5mL slurry transferred into anaerobic Widdel medium after 372 days
source material identifiers
true
submitted to insdc
protein, antibiotics, sulfate
substrated added experiment
V4
target subfragment
Helgoland mud area slurry incubation - protein.antibiotics.sulfate - day 98
title
28.8
m
total depth of water column