2024-10-25
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115078299
External Id
Comisión Nacional de Energía Atómica
INSDC center name
2024-01-02T11:16:01Z
INSDC last update
public
INSDC status
ERS17692038
SRA accession
E-MTAB-13681:Sample 5
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Mid-exponential cultures (OD650 0.3) were diluted to OD650 0.05 in LB medium and divided into two 30 ml fractions, each of which was placed in a glass beaker (4.5 cm internal diameter). The beakers were placed in a multichamber coupled to a thermocycler bath so that the temperature of the suspensions was maintained at 37°C. One of the fractions was irradiated from above at an irradiance of 25 W m2 at the level of the free surface of the suspension, whilst the other fraction was covered with a black plastic sheet (dark control). The cell suspensions were stirred continuously with a magnetic bar. Cells grown under UVA and their respective controls (see above) were harvested OD650 0.3 and total RNA was extracted by using an RNA extraction kit (Ambion-Life Technology). The library was prepared with TruSeq Stranded Total RNA with Ribo-Zero beast to remove ribosomar RNA
description
not collected
geographic location (country and/or sea)
early logaritmic
growth condition
not applicable
isolate
Pseudomonas aeruginosa
organism
Pseudomonas aeruginosa
organism
whole organism
organism part
Pseudomonas aeruginosa
scientific_name
UV-sublethal dose
stimulus
PAO1
strain
Sample 5
title