2024-10-28
ENA first public
ERC000011
ENA-CHECKLIST
University of Wrocław, Faculty of Biotechnology
INSDC center name
public
INSDC status
ERS18345380
SRA accession
E-MTAB-13846:Sample 8
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: For RNA-seq, total RNA was isolated from 30 ml YEME/TSB cultures. Cultures were inoculated with S. coelicolor and cultivated in flasks with spring coils for 24-36h in 30°C. For osmotic stress experiment growth medium was supplemented with 0.5M NaCl. Mycelia were collected at two time points (exponential and early stationary growth, determined individually for each strain based on the growth curve (Fig. S3)) by collecting 2 ml from the culture and centrifugation. Cell pellets were frozen and stored at -70°C for subsequent RNA isolation.  RNA was isolated using the RNeasy Mini Kit (Qiagen) following manufacturer’s instructions, DNA digestions was performed using on column digestion with DNase-I (Qiagen) and TURBO DNase I (Ambion). RNA quality and concentration was measured using Nanodrop (Thermo Fisher Scientific) and Qubit (Thermo Fisher Scientific).  The absence of DNA in the sample was confirmed using PCR. Library preparation was performed by Genewiz (Germany).
description
exponential
developmental stage
dhupA
genotype
not collected
geographic location (country and/or sea)
standard medium
growth condition
not applicable
isolate
Streptomyces coelicolor
organism
Streptomyces coelicolor
organism
whole organism
organism part
Streptomyces coelicolor
scientific_name
A3(2)
strain
Sample 8
title