2024-10-28
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA111529176
External Id
Germline Stem Cells and Microenvironment Lab, College of Animal Science and Technology, Nanjing Agricultural University
INSDC center name
2022-10-28T17:31:02Z
INSDC last update
public
INSDC status
ERS13639157
SRA accession
E-MTAB-12342:Sample 8
Submitter Id
2 to 3
day
age
ArrayExpress
broker name
germline stem cell derived pluripotent cell
cell_type
house mouse
common name
Protocols: Around 1x105 SSCs (cultured in medium 1 for 7 passages), Intermediate state cells (SSCs cultured in medium 1 for 5 passages and further cultured in medium 2 for 2 passages), GSPCs (SSCs cultured in medium 1 for 5 passages and further cultured in medium 2 for 5 passages) and ES-like cells (10 passages) were collected using the identical protocols. Total RNA was extracted using Trizol (Ambion Life Technologies) according to the Ambion standard RNA isolation procedure. mRNA was purified using the NEBNext Poly (A) mRNA Magnetic Isolation Beads (NEB, USA). Then the mRNA library was constructed with a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420S/L, NEB).
description
mouse postnatal
developmental stage
wild type genotype
genotype
GSPCs medium
growth condition
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
Mus musculus
scientific_name
ICR
strain
Sample 8
title