2024-10-30
ENA first public
ERC000011
ENA-CHECKLIST
Department of Pharmacology and Pharmacotherapy, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary
INSDC center name
public
INSDC status
ERS18431548
SRA accession
E-MTAB-13909:Sample 12
Submitter Id
3
month
age
ArrayExpress
broker name
not collected
collection date
Norway rat
common name
Protocols: The hearts of the animals were removed and subjected to Langendorff perfusion for 2 minutes at 37°C. During this perfusion, the hearts were flushed with oxygenated Krebs-Henseleit solution (in millimolar (mM): NaCl 118, KCl 4.7, MgSO4 1.2, CaCl2 1.25, KH2PO4 1.2, NaHCO3 25, and glucose 11) to eliminate any remaining blood from the tissue. Afterwards, the samples were snap-frozen in liquid nitrogen and stored at -80 °C.  For in vivo experiments, two-month-old male Wistar rats of 189–287 g were randomised and treated daily for 28 ± 1 days by oral gavage with 5.12 mg kg−1 rofecoxib or with its vehicle (1% hydroxyethylcellulose).  The total RNA of the left ventricle tissue samples (n = 8/group) was extracted by employing the RNAzol RT kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer´s instructions. RNA concentrations of all samples were quantified using spectrophotometry (NanoDrop; Thermo Fischer Scientific, Waltham, MA, USA) and RNA HS Assay Kit with Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). The RNA Integrity Numbers were determined by 2100 Bioanalyzer Instrument (Agilent Technologies, Santa Clara, CA, USA) and agarose gel electrophoresis. We employed the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA, USA) for small RNA library construction according to the manufacturer’s protocol. Libraries were quantified and qualified using High Sensitivity DNA1000 ScreenTape system with 2200 Tapestation (Agilent Technologies, Santa Clara, CA, USA) and dsDNA HS Assay Kit with Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).
description
adult
developmental stage
wild type genotype
genotype
not collected
geographic location (country and/or sea)
204_vehicle
individual
not applicable
isolate
Rattus norvegicus
organism
Rattus norvegicus
organism
heart left ventricle
organism part
Rattus norvegicus
scientific_name
male
sex
WIstar
strain
Sample 12
title