2024-10-31
ENA first public
ERC000011
ENA-CHECKLIST
Vanderbilt University
INSDC center name
public
INSDC status
ERS18708930
SRA accession
E-MTAB-14000_2:9397-LT-1
Submitter Id
ArrayExpress
broker name
endodermal cell
cell_type
not collected
collection date
house mouse
common name
Protocols: Timed matings between CD1 wild type mice or between Sox17Δ50/Δ50 mice were performed. Wild type and Sox17Δ50/Δ50 embryos were collected separately at E10.5 on ice-cold PBS. Posterior foregut and midgut regions of embryos were dissected by removal of embryonic head, heart, tail, limb buds, posterior and dorsal body trunk. Dissected embryonic tissues were pooled according to genotype and dissociated into single cells by incubating and regular pipetting in Accumax (Millipore Sigma, #A7089) supplemented with DNase I  for 15 minutes at 37C Wild type and Sox17Δ50/Δ50 cell samples were stained separately with Cell Multiplexing Oligo (10x Genomics 3’ CellPlex Kit) according to manufacturer recommendations, counted and pooled together at 1:1 ratio in PBS containing 10% FBS on ice. DAPI staining was performed 15 minutes before sorting to enrich live cells at Vanderbilt Flow Cytometry Shared Resource. Library preparation and sequencing was carried out using Chromium Single Cell 3' Reagent Kits v3.1. Libraries were constructed according to the manufacturer’s instructions for 10x Genomics Single Cell 3′ Library  kit v3
description
E10.5
developmental stage
mixed - Sox17∆50/∆50 and Sox17+/+
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Mus musculus
organism
Mus musculus
organism
embryonic foregut
organism part
Mus musculus
scientific_name
CD1
strain
9397-LT-1
title