2024-11-01
ENA first public
ERC000011
ENA-CHECKLIST
Molecular Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
INSDC center name
public
INSDC status
ERS21188114
SRA accession
E-MTAB-14511:DMSO1
Submitter Id
ArrayExpress
broker name
AGS
cell_line
epithelial
cell_type
not collected
collection date
human
common name
Protocols: AGS cells were digested with trypsin, then were neutralized and collected.  Human gastric cancer cell line AGS was cultured in DMEM high-glucose culture medium containing 10%FBS, placed in a 5% CO2 incubator at 37 ℃, digested with 0.25% trypsin every 3 to 4 days, and passed through 1:3 cells with logarithmic growth phase for experiment. AGS cells treated with capsaicin (at a dose of 250uM for 24h) or DMSO Total RNA was collected from AGS cells treated with capsaicin or DMSO using TRIzol reagent according to the manufacturer’s protocol (n=3 per group). RNA was quantified using a NanoDrop ND-2000 (Thermo Scientific, USA), and RNA integrity was assessed using an Agilent Bioanalyzer 2100 (Agilent Technologies, USA).  Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.
description
adult
developmental stage
gastric carcinoma
disease
wild type genotype
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
stomach
organism part
Homo sapiens
scientific_name
DMSO1
title