2024-11-01
ENA first public
ERC000011
ENA-CHECKLIST
Institutionen för molekylärbiologi Institutionen för molekylärbiologi, 90187 Umeå
INSDC center name
public
INSDC status
ERS20223271
SRA accession
E-MTAB-14158:Op_803_2
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Twenty young adult animals were picked to the fresh plates and allowed to lay eggs for 2 hours, after which the adult animals were removed and eggs were allowed to develop into L4 stage. Ten plates of L4 animals were washed off and placed on either OP50 or V. cholerae containing plates for 8 hours. Subsequently, the animals were collected and rinsed three times with M9 buffer. After washing, the worm pellet was frozen in liquid nitrogen. Animals were homogenized using Bullet Blender (Next Advance) in the presence of Qiazol Lysis Reagent and 0.5mm Zirconia beads at 4°C. RNA was prepared using RNeasy Plus Universal Mini Kit (Qiagen). Library was constructed and sequenced by Novogene
description
L4 larva
developmental stage
shk-1 KO
genotype
not collected
geographic location (country and/or sea)
mixed
individual
none
infect
not applicable
isolate
Caenorhabditis elegans
organism
Caenorhabditis elegans
organism
whole organism
organism part
Caenorhabditis elegans
scientific_name
mixed
sex
Op_803_2
title