2024-11-01
ENA first public
ERC000011
ENA-CHECKLIST
Lausanne University Hospital
INSDC center name
public
INSDC status
ERS21362013
SRA accession
E-MTAB-14560:Chromium_DLBCL_breast
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Curls with 2x25 micrometers for lung, breast and DLBCL were sectioned from FFPE blocks. Nuclei were extracted using snPATHO protocol9, based on the protocol from 10X (CG000632). The nuclei were counted on the LUNA-FX7 cell counter (AO/PI viability kit, Logos). Pools of four samples were processed together as shown in Supplementary Table 4, targeting 40,000 nuclei recovery per pool (10,000 nuclei per sample). Replicas for two donors B3 and B4 were processed independently. The protocol was followed by the manufacturer's protocol Chromium Fixed RNA profiling - Multiplexed samples (CG000527).
description
diffuse large B-cell lymphoma; breast cancer
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
Chromium_DLBCL_breast
title