2024-11-01
ENA first public
ERC000011
ENA-CHECKLIST
SAMEA115116643
External Id
Department of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Kyoto, Japan
INSDC center name
2024-01-18T13:52:28Z
INSDC last update
public
INSDC status
ERS17754795
SRA accession
E-MTAB-13701:gRNA8-2
Submitter Id
ArrayExpress
broker name
not collected
collection date
brewer's yeast
common name
Protocols: Cultured yeast cells were used to isolate mitochondria using a Yeast Mitochondria Isolation Kit (Abcam). mtDNA was extracted from isolated mitochondria using the FastGene Plasmid Mini Kit (Nippon Genetics, Tokyo, Japan) and purified using AMpure XP beads (Beckman Coulter, CA, USA). Extracted gDNA and mtDNA were subjected to library construction using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA).
description
mitochondrial fraction
fraction
CEN.PK2-1C (Euroscarf: 30016A) pMnC-CDA p426-gRNA-8
genotype
not collected
geographic location (country and/or sea)
YPG (48 hours)
growth condition
not applicable
isolate
Saccharomyces cerevisiae
organism
Saccharomyces cerevisiae
organism
whole organism
organism part
Saccharomyces cerevisiae
scientific_name
S288c
strain
gRNA8-2
title