2024-11-01
ENA first public
ERC000025
ENA-CHECKLIST
SAMEA111538040
External Id
Thuenen-Institute of Biodiversity
INSDC center name
2022-11-01T15:52:26Z
INSDC last update
public
INSDC status
ERS13648073
SRA accession
F-HEAD-11-R-TAIL-1-B
Submitter Id
cropland biome
broad-scale environmental context
2020
collection date
swab; parasitized nest; 10 brood cells; short fragment; Replicate B
description
nesting material
environmental medium
Germany
geographic location (country and/or sea)
52.28390526
DD
geographic location (latitude)
10.44338024
DD
geographic location (longitude)
nest tube of cavity-nesting hymenoptera
local environmental context
FWD: AAATCTT; REV: TCTACTG
multiplex identifiers
PCR
nucleic acid amplification
ZymoBIOMICS DNA Microprep Kit
nucleic acid extraction
insect metagenome
organism
FWD: GCTATGCGCGAGCtgcGGDACWGGWTGAACWGTWTAYCCHCC; REV: GCGAGCTGCGCTAtgcGTRATWGCHCCDGCTARWACWGG
pcr primers
eDNA cavity-nesting wild bees
project name
feces: collected from nest tube, stored at -20�C, swabs: collected using DNA-free swab in PBS, stored at -20�C
sample material processing
insect metagenome
scientific_name
Illumina MiSeq
sequencing method
COI
target gene
PC3-B
title