2024-11-05
ENA first public
ERC000011
ENA-CHECKLIST
Department of Hematology cancer institute ErasmusMC
INSDC center name
public
INSDC status
ERS19896183
SRA accession
E-MTAB-14075:WT_young
Submitter Id
12
month
age
ArrayExpress
broker name
hematopoietic cell
cell_type
not collected
collection date
zebrafish
common name
Protocols: Adult zebrafish were euthanized, kidney marrow was isolated and dissociated by pipetting in PBS/10% FCS. 7-AAD (7-amino-actinomycin D) 0.5mg/L (BD biosciences) was used for live/dead discrimination. Sorting was performed using FACSAriaIII (BD). 70.000 single viable cells were sorted from 2 pooled kidney marrow of female Tg(CD41:GFP) zebrafish and supplemented with 114-1607 CD41:GFPlow expressing cells. cDNA was prepared using the manufacturers protocol (10x Chromium V3). Library was prepared using the manufacturers protocol (10x Chromium V3).
description
adult
developmental stage
Gata2bWT
genotype
not collected
geographic location (country and/or sea)
pools of 2 fish
individual
not applicable
isolate
Danio rerio
organism
Danio rerio
organism
kidney marrow
organism part
Danio rerio
scientific_name
mixed
sex
Tg(CD41:GFP; runx1:DsRed) WT
strain
WT_young
title