2024-11-06
ENA first public
ERC000011
ENA-CHECKLIST
Centre for Genomic Regulation (CRG)
INSDC center name
public
INSDC status
ERS21361941
SRA accession
E-MTAB-14562:CapTrap_HpreCap_Placenta_GENCODE_pbSII
Submitter Id
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Commercial samples obtained from Ambion (Thermo Fisher Scientific) or Clontech-Takara Bio: Both Ambion and Clontech-Takara Bio are brands specializing in life science research products, with a focus on RNA technologies. Ambion is a brand owned by Thermo Fisher Scientific, a global provider of scientific research products and services, while Clontech is part of Takara Bio, known for molecular biology tools. Therefore, \"commercial samples obtained from Ambion (Thermo Fisher Scientific) or Clontech-Takara Bio\" refers to RNA samples purchased from these brands for research purposes. Total RNA was extracted from the collected samples using protocols performed by commercial providers Ambion (Thermo Fisher Scientific) or Clontech-Takara Bio. Although the exact kit or extraction protocol used was not specified, the RNA extraction followed the manufacturers' standard procedures, ensuring high-quality and reliable RNA yield for downstream applications. CapTrap cDNA library preparation: CapTrap cDNA library preparation is a long-read sequencing protocol that selectively enriches 5'-capped transcripts by capturing the 5' caps of transcripts. This protocol uses Poly(A) tail selection to specifically target mRNA, and a 5' cap-specific oxidation reaction coupled to biotin-streptavidin to capture the 5' cap structure. The captured mRNA is then converted to cDNA and the library is prepared with 5' and 3' specific linkers to enrich for full-length libraries. CapTrap is particularly useful for studying transcription start sites, alternative splicing, and post-transcriptional modifications.
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
PacBio
sequencing
CapTrap_HpreCap_Placenta_GENCODE_pbSII
title