2024-11-07
ENA first public
ERC000011
ENA-CHECKLIST
NTU
INSDC center name
public
INSDC status
ERS20963367
SRA accession
E-MTAB-14439:ST11
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Bacteria were harvested by centrifugation and the cell pellets were submerged in Trizol and shipped on dry ice to Macrogen Inc. (South Korea) for RNA extraction and sequencing. Samples were relabelled in a randomised order before sending to the sequencing provider, to avoid any potential for introduction of a technical batch effect based on which group (treated or untreated) the samples belonged to. Total RNA extraction was conducted by Macrogen Inc. (South Korea). Total RNA was quality checked to confirm that all were of acceptable RNA Integrity Number (RIN >7). Libraries were constructed using the Illumina TruSeq Stranded Total RNA (Illumina Inc, San Diego) protocol with ribosomal RNA depletion (Ribo-Zero).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Helicobacter pylori
organism
Helicobacter pylori
organism
whole organism
organism part
Helicobacter pylori
scientific_name
ST11
title