2024-11-11
ENA first public
ERC000011
ENA-CHECKLIST
Newcastle University Wellcome Sanger Institute
INSDC center name
public
INSDC status
ERS21345226
SRA accession
E-MTAB-12303:CTCL8_Derm45N_GEX_S1
Submitter Id
61
year
age
ArrayExpress
broker name
not collected
collection date
human
common name
Protocols: Samples were collected as biopsies from patients who have CTCL. Each biopsy was then digested into single cells by firstly separating the epidermis and dermis using dispase and then incubating each section with collagenase. Cells were FACS sorted before loading onto the 10x Chromium. During droplet encapsulation the cells are lyse. cDNA is made from the RNA of each cell by reverse transcription and then purified using Dyna beads. Libraries are constructed as per the 10x Genomics protocol: \"Enzymatic fragmentation and size selection are used to optimize the cDNA amplicon size prior to 5’ Gene Expression library construction. P5, P7, a sample index, and Illumina R2 sequence (read 2 primer sequence) are added via End Repair, A-tailing, Adaptor Ligation, and Sample Index PCR. The final libraries contain the P5 and P7 priming sites used in Illumina sequencers.\"
description
adult
developmental stage
Cutaneous T-cell lymphoma
disease
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
skin of body
organism part
8
replicate
Homo sapiens
scientific_name
male
sex
CTCL8_Derm45N_GEX_S1
title