2024-11-11
ENA first public
ERC000021
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS16590020
SRA accession
EMOBON00085
Submitter Id
marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
broad-scale environmental context
2021-07-26
collection date
22.94
m
depth
EMOBON metagenome sediment sample from station BPNS collected on 2021-07-26 community collected micro
description
5.33
ng/μL
dna concentration
-22.94
m
elevation
sediment [ENVO:00002007]
environmental medium
Belgium
geographic location (country and/or sea)
51.433331
DD
geographic location (latitude)
Belgian part of the North Sea
geographic location (locality)
2.808331
DD
geographic location (longitude)
North Atlantic Ocean
geographic location (ocean)
North Sea
geographic location (region and locality)
43017431.0
library reads sequenced
shallow_marine_sediment_[ENVO:03000034]
local environmental context
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
nucleic acid extraction
BPNS
obs_id
marine sediment metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
220712_JARVIS_HMNJKDSX3
run
Van Veen Grab
sample collection device
SoSOP3
sample collection method
SoSOP3
sample material processing
MSO LifeWatch Lab -80 Freezer
sample storage location
-80
°C
sample storage temperature
marine sediment metagenome
scientific_name
°C
sea surface temperature
no CTD taken
sea surface temperature method
°C
sediment temperature
no CTD taken
sediment temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220712
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
EMOBON_BPNS_So_210726_micro_2
source material identifiers
sample
status
metag
target gene
metag
target subfragment
high
tidal stage
flood_tide
tidal stage information
EMOBON_BPNS_So_6
title
26
m
total depth of water column