2024-11-11
ENA first public
ERC000021
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20535377
SRA accession
EMOBON00092
Submitter Id
marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
broad-scale environmental context
21.69
µg/L
chlorophyll
Fluorometer 10-AU
chlorophyll method
2021-08-05
collection date
0
m
depth
EMOBON metagenome sediment sample from station RFormosa collected on 2021-08-05 community collected micro
description
271.875
µmol/kg
dissolved oxygen
YSI ProSolo Digital
dissolved oxygen method
9.05
ng/µl
dna concentration
0
m
elevation
lagoon [ENVO_00000038]; sediment [ENVO:00002007]
environmental medium
Portugal
geographic location (country and/or sea)
37.005639
DD
geographic location (latitude)
Ria Formosa
geographic location (locality)
-7.96925
DD
geographic location (longitude)
Atlantic Ocean
geographic location (ocean)
North Atlantic Ocean
geographic location (region and locality)
61701779
library reads sequenced
lagoon [ENVO_00000038];shallow_marine_sediment [ENVO:03000034]
local environmental context
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
nucleic acid extraction
RFormosa
obs_id
marine sediment metagenome
organism
7.9
pH
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
Thermo scientific  orion star A221
ph method
0.998766
atm
pressure
YSI ProSolo Digital
pressure method
EMOBON
project name
-101.7
mV
redox potential
Thermo scientific  orion star A221
redox potential method
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
38.03
psu
salinity
YSI ProSolo Digital
salinity method
PVC core D50mm
sample collection device
SoSOP 1
sample material processing
Ed7, room 1.10, freezer #300387-118
sample storage location
-80
°C
sample storage temperature
marine sediment metagenome
scientific_name
26.3
ºC
sea surface temperature
Thermo scientific  orion star A221
sea surface temperature method
24
ºC
sediment temperature
Thermo scientific  orion star A221
sediment temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220712
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
EMOBON_RFormosa_So_210805_micro_1
source material identifiers
sample
status
metag
target gene
metag
target subfragment
low
tidal stage
low_tide
tidal stage information
EMOBON_RFormosa_So_1
title
0
m
total depth of water column