2024-11-11
ENA first public
ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20568987
SRA accession
EMOBON00072
Submitter Id
30
L
amount or size of sample collected
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023];mediterranean sea biome[ENVO_01000047]
broad-scale environmental context
0.236
µg/L
chlorophyll
TD-700 Laboratory Fluorometer
chlorophyll method
2021-06-28
collection date
1
m
depth
EMOBON metagenome water sample from station HCMR-1 collected on 2021-06-28 size fraction 0.2-3um
description
8.05
ng/µl
dna concentration
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]
environmental medium
Greece
geographic location (country and/or sea)
35.346621
DD
geographic location (latitude)
Crete Sea
geographic location (locality)
25.278761
DD
geographic location (longitude)
Mediterranean Sea - Eastern Basin
geographic location (ocean)
Aegean Sea
geographic location (region and locality)
46200567
library reads sequenced
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
local environmental context
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
HCMR-1
obs_id
marine plankton metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
39.4
psu
salinity
HQ1140 Portable Conductivity/TDS Meter with Conductivity Electrode
salinity method
Jerrycan
sample collection device
WaSOP 1
sample material processing
Basement, New chest freezer -80C, Left side, Carton box
sample storage location
-80
°C
sample storage temperature
marine plankton metagenome
scientific_name
27.7
ºC
sea subsurface temperature
HQ1140 Portable Conductivity/TDS Meter with Conductivity Electrode
sea subsurface temperature method
27.7
ºC
sea surface temperature
HQ1140 Portable Conductivity/TDS Meter with Conductivity Electrode
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220712
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
0.2
µm
size-fraction lower threshold
3
µm
size-fraction upper threshold
EMOBON_HCMR-1_Wa_210628_0.2um_2
source material identifiers
sample
status
39.4
psu
subsurface salinity
HQ1140 Portable Conductivity/TDS Meter with Conductivity Electrode
subsurface salinity method
metag
target gene
metag
target subfragment
no_tide
tidal stage information
EMOBON_HCMR-1_Wa_6
title
21
m
total depth of water column