2024-11-11
ENA first public
ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20569058
SRA accession
EMOBON00004
Submitter Id
10
L
amount or size of sample collected
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023]
broad-scale environmental context
0.821
µg/L
chlorophyll
photometric measurement
chlorophyll method
2021-08-30
collection date
2
m
depth
EMOBON metagenome water sample from station Bergen collected on 2021-08-30 size fraction 3-200um
description
5.05
ng/µl
dna concentration
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
environmental medium
Norway
geographic location (country and/or sea)
68.925895
DD
geographic location (latitude)
Norwegian part of the Norwegian Sea
geographic location (locality)
17.125619
DD
geographic location (longitude)
Arctic Ocean
geographic location (ocean)
Norwegian Sea
geographic location (region and locality)
81370157
library reads sequenced
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049];fjord [ENVO_00000039]
local environmental context
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_eu
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
ESC68N
obs_id
marine plankton metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
32.5
psu
salinity
CTD-system
salinity method
Niskin
sample collection device
WaSOP1
sample material processing
eDNA freezer bag
sample storage location
-80
°C
sample storage temperature
marine plankton metagenome
scientific_name
11.67
ºC
sea subsurface temperature
CTD-system
sea subsurface temperature method
11.69
ºC
sea surface temperature
CTD-system
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220622
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
3
µm
size-fraction lower threshold
200
µm
size-fraction upper threshold
EMOBON_ESC68N_Wa_210830_3um_2
source material identifiers
sample
status
32.5
psu
subsurface salinity
CTD-system
subsurface salinity method
metag
target gene
metag
target subfragment
no_tide
tidal stage information
EMOBON_ESC68N_Wa_2
title