2024-11-11
ENA first public
ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20569047
SRA accession
EMOBON00028
Submitter Id
13
L
amount or size of sample collected
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023];mediterranean sea biome[ENVO_01000047]
broad-scale environmental context
2021-08-23
collection date
1
m
depth
EMOBON metagenome water sample from station VB collected on 2021-08-23 size fraction 3-9999um
description
1.075
ng/µl
dna concentration
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
environmental medium
France
geographic location (country and/or sea)
43.683
DD
geographic location (latitude)
Villefranche Bay - Point B
geographic location (locality)
7.317
DD
geographic location (longitude)
Mediterranean Sea - Western Basin
geographic location (ocean)
Villefranche Bay
geographic location (region and locality)
68846432
library reads sequenced
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
local environmental context
DDC_AAABOSDA_1_HWLTKDRXY.UDI213
mock_eu
DDC_AAAAOSDA_1_HWLTKDRXY.UDI212
mock_pr
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
VB
obs_id
marine plankton metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:11
pcr conditions
metag
pcr primers
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
Niskin
sample collection device
WaSOP 1
sample material processing
-80°C galeriens 1st shelf left
sample storage location
-80
°C
sample storage temperature
marine plankton metagenome
scientific_name
manual
sequence quality check
Batch1
sequence_batch
Batch1_220223
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
3
µm
size-fraction lower threshold
200
µm
size-fraction upper threshold
EMOBON_VB_Wa_210823_3um_2
source material identifiers
sample
status
metag
target gene
metag
target subfragment
no_tide
tidal stage information
EMOBON_VB_Wa_42
title
80
m
total depth of water column