2024-11-11
ENA first public
ERC000021
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20535367
SRA accession
EMOBON00088
Submitter Id
marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
broad-scale environmental context
2021-08-26
collection date
17
m
depth
EMOBON metagenome sediment sample from station ROSKOGO collected on 2021-08-26 community collected micro
description
48.3
ng/µl
dna concentration
-17
m
elevation
sediment [ENVO:00002007]
environmental medium
France
geographic location (country and/or sea)
48.708333
DD
geographic location (latitude)
French part of the English Channel
geographic location (locality)
-3.866
DD
geographic location (longitude)
North Atlantic Ocean
geographic location (ocean)
English Channel
geographic location (region and locality)
49808546
library reads sequenced
shallow_marine_sediment_[ENVO:03000034]
local environmental context
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
nucleic acid extraction
ROSKOGO
obs_id
marine sediment metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
core
sample collection device
SoSOP2
sample material processing
SBR YD001 freezer 17
sample storage location
-80
°C
sample storage temperature
marine sediment metagenome
scientific_name
17
ºC
sea surface temperature
deduced from temperature measured at Estacade on 1/9/2021
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220712
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
EMOBON_ROSKOGO_So_210826_micro_1
source material identifiers
sample
status
metag
target gene
metag
target subfragment
low
tidal stage
ebb_tide
tidal stage information
EMOBON_ROSKOGO_So_1
title
18
m
total depth of water column