2024-11-11
ENA first public
ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20569005
SRA accession
EMOBON00045
Submitter Id
4.57
µmol/L
ammonium
Spectrophotometry
ammonium method
5.4
L
amount or size of sample collected
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023];estuarine biome [ENVO:01000020]
broad-scale environmental context
2.62
µg/L
chlorophyll
Spectrophotometry
chlorophyll method
2021-08-31
collection date
9642.9
mS/cm
conductivity
in situ multiparametric probe
conductivity method
1
m
depth
EMOBON metagenome water sample from station OSD74 collected on 2021-08-31 size fraction 200um
description
7.84
µmol/kg
dissolved oxygen
in situ multiparametric probe
dissolved oxygen method
6.69
ng/µl
dna concentration
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
environmental medium
Portugal
geographic location (country and/or sea)
41.146528
DD
geographic location (latitude)
Porto Valley
geographic location (locality)
-8.666639
DD
geographic location (longitude)
Atlantic Ocean
geographic location (ocean)
North Atlantic Ocean
geographic location (region and locality)
86482865
library reads sequenced
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
local environmental context
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_eu
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
mock_pr
32.25
µmol/L
nitrate
Spectrophotometry
nitrate method
1.15
µmol/L
nitrite
Spectrophotometry
nitrite method
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
OSD74
obs_id
marine plankton metagenome
organism
7.32
pH
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
in situ multiparametric probe
ph method
1.83
µg/L
phaeopigments
Spectrophotometry
phaeopigments method
0.52
µmol/L
phosphate
Spectrophotometry
phosphate method
0.127
atm
pressure
in situ multiparametric probe
pressure method
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
5.42
psu
salinity
in situ multiparametric probe
salinity method
Bucket
sample collection device
WaSOP1
sample material processing
CIIMAR - EcoBioTec New -80ºC Freezer
sample storage location
-80
°C
sample storage temperature
marine plankton metagenome
scientific_name
22.745
ºC
sea subsurface temperature
in situ multiparametric probe
sea subsurface temperature method
22.724
ºC
sea surface temperature
in situ multiparametric probe
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220622
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
45.36
µmol/L
silicate
Spectrophotometry
silicate method
3
µm
size-fraction lower threshold
200
µm
size-fraction upper threshold
EMOBON_OSD74_Wa_210831_3um_1
source material identifiers
sample
status
5.31
psu
subsurface salinity
in situ multiparametric probe
subsurface salinity method
metag
target gene
metag
target subfragment
high
tidal stage
high_tide
tidal stage information
EMOBON_OSD74_Wa_1
title
1
m
total depth of water column
0.67
FTU
turbidity