2024-11-11
ENA first public
ERC000021
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20535387
SRA accession
EMOBON00095
Submitter Id
marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
broad-scale environmental context
2021-06-21
collection date
75
m
depth
EMOBON metagenome sediment sample from station NRMCB collected on 2021-06-21 community collected micro
description
4.741
µmol/kg
dissolved oxygen
SBE43
dissolved oxygen method
21.9
ng/µl
dna concentration
-75
m
elevation
sediment [ENVO:00002007]
environmental medium
Italy
geographic location (country and/or sea)
40.800139
DD
geographic location (latitude)
Naples Gulf
geographic location (locality)
14.25
DD
geographic location (longitude)
Mediterranean Sea - Western Basin
geographic location (ocean)
Tyrrhenian Sea
geographic location (region and locality)
49770551
library reads sequenced
shallow_marine_sediment_[ENVO:03000034]
local environmental context
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
nucleic acid extraction
NRMCB
obs_id
marine sediment metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
0.5523
µg/L
pigments
this is not pigments but fluorescense. chl Turner Design Cyclops 7C)
pigments method
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
37.5864
psu
salinity
CTD SBE 19plusV2
salinity method
sediment corer
sample collection device
So SOP1
sample material processing
freezer #1 BEOM; upper compartment; rack #8 d III (see scheme in technical support EMI)
sample storage location
-80
°C
sample storage temperature
marine sediment metagenome
scientific_name
25.0495
ºC
sea surface temperature
CTD SBE 19plusV2
sea surface temperature method
14.999
ºC
sediment temperature
CTD SBE 19plusV2
sediment temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220712
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
EMOBON_NRMCB_So_210621_micro_2
source material identifiers
sample
status
metag
target gene
metag
target subfragment
no_tide
tidal stage information
EMOBON_NRMCB_So_2
title
75
m
total depth of water column