2024-11-11
ENA first public
ERC000021
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20535390
SRA accession
EMOBON00099
Submitter Id
Control
broad-scale environmental context
2021-08-31
collection date
0
m
depth
EMOBON metagenome sediment sample from station NRMCB collected on 2021-08-31 community collected micro
description
0
m
elevation
swab
environmental medium
missing: control sample
geographic location (country and/or sea)
40.800139
DD
geographic location (latitude)
Naples Gulf
geographic location (locality)
14.25
DD
geographic location (longitude)
Mediterranean Sea - Western Basin
geographic location (ocean)
Tyrrhenian Sea
geographic location (region and locality)
43917269
library reads sequenced
Negative
local environmental context
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
nucleic acid extraction
NRMCB
obs_id
metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:2;Nocycles:18
pcr conditions
metag
pcr primers
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
freezer #1 BEOM; upper compartment; rack #8 d III (see scheme in technical support EMI)
sample storage location
-80
°C
sample storage temperature
metagenome
scientific_name
manual
sequence quality check
Batch1
sequence_batch
Batch1_220712
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
EMOBON_NRMCB_So_210831_micro_blank
source material identifiers
blank
status
metag
target gene
metag
target subfragment
no_tide
tidal stage information
EMOBON_NRMCB_So_10
title
75
m
total depth of water column