2024-11-11
ENA first public
ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20568976
SRA accession
EMOBON00083
Submitter Id
10
L
amount or size of sample collected
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023]
broad-scale environmental context
0.094167
µg/L
chlorophyll
Trilogy fluorometer - Module: CHL-A NA model # 7200-046
chlorophyll method
2021-08-29
collection date
2
m
depth
EMOBON metagenome water sample from station IUIEilat collected on 2021-08-29 size fraction 3-200um
description
1.92
ng/µl
dna concentration
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
environmental medium
Israel
geographic location (country and/or sea)
29.5
DD
geographic location (latitude)
Gulf of Eilat
geographic location (locality)
34.916667
DD
geographic location (longitude)
Indian Ocean
geographic location (ocean)
Gulf of Eilat
geographic location (region and locality)
83595057
library reads sequenced
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
local environmental context
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_eu
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
IUIEilat
obs_id
marine plankton metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
Hand pump
sample collection device
WaSOP 1
sample material processing
Eppendorf F570 -80 freezer
sample storage location
-80
°C
sample storage temperature
marine plankton metagenome
scientific_name
27.4
ºC
sea surface temperature
Thermistor - Campbell Scientific  108
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220622
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
3
µm
size-fraction lower threshold
200
µm
size-fraction upper threshold
EMOBON_IUIEilat1_Wa_210829_3um_2
source material identifiers
sample
status
40.7649
psu
subsurface salinity
MS-315e Salinometer
subsurface salinity method
metag
target gene
metag
target subfragment
no_tide
tidal stage information
EMOBON_IUIEilat_Wa_6
title
700
m
total depth of water column