2024-11-11
ENA first public
ERC000021
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20535383
SRA accession
EMOBON00001
Submitter Id
marine biome [ENVO:00000447];marine benthic biome[ENVO_01000024]
broad-scale environmental context
2021-06-08
collection date
27
m
depth
EMOBON metagenome sediment sample from station OOB collected on 2021-06-08 community collected micro
description
1.7
ng/µl
dna concentration
-27
m
elevation
sediment [ENVO:00002007]
environmental medium
France
geographic location (country and/or sea)
42.484167
DD
geographic location (latitude)
Bay of Banyuls-sur-Mer
geographic location (locality)
3.135278
DD
geographic location (longitude)
Mediterranean Sea - Western Basin
geographic location (ocean)
Gulf of Lion
geographic location (region and locality)
58079642
library reads sequenced
shallow_marine_sediment_[ENVO:03000034]
local environmental context
DDC_AAABOSDA_1_HWLTKDRXY.UDI213
mock_eu
DDC_AAAAOSDA_1_HWLTKDRXY.UDI212
mock_pr
metag
nucleic acid amplification
https://www.qiagen.com/us/products/discovery-and-translational-research/dna-rna-purification/dna-purification/microbial-dna/dneasy-powersoil-pro-kit?catno=47016
nucleic acid extraction
OOB
obs_id
marine sediment metagenome
organism
7.962
pH
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
Consort multimeter C3016
ph method
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
37.85
psu
salinity
Seabird SBE 911plus CTD
salinity method
PVC sediment corer
sample collection device
SoSOP 2
sample material processing
room 213, freezer LECOB
sample storage location
-80
°C
sample storage temperature
marine sediment metagenome
scientific_name
18.75
ºC
sea surface temperature
Seabird SBE 911plus CTD
sea surface temperature method
18.66
ºC
sediment temperature
Consort temperature compensator (ST10) PT1000
sediment temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220223
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
EMOBON_OOB_So_210608_micro_1
source material identifiers
sample
status
metag
target gene
metag
target subfragment
no_tide
tidal stage information
EMOBON_OOB_So_1
title
27
m
total depth of water column