2024-11-11
ENA first public
ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20568991
SRA accession
EMOBON00049
Submitter Id
0.53
µmol/L
ammonium
5
L
amount or size of sample collected
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023];mediterranean sea biome[ENVO_01000047]
broad-scale environmental context
1.13
µg/L
chlorophyll
filtration on 0.2 um FG filters. extraction in acetone overnight. fluorimetric measurement
chlorophyll method
2021-06-22
collection date
1
m
depth
EMOBON metagenome water sample from station AAOT collected on 2021-06-22 size fraction 3-200um
description
9.09
ng/µl
dna concentration
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
environmental medium
Italy
geographic location (country and/or sea)
45.314167
DD
geographic location (latitude)
Gulf of Venice
geographic location (locality)
12.508333
DD
geographic location (longitude)
Mediterranean Sea - Eastern Basin
geographic location (ocean)
Adriatic Sea
geographic location (region and locality)
78700756
library reads sequenced
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
local environmental context
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_eu
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
mock_pr
1.08
µmol/L
nitrate
0.18
µmol/L
nitrite
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
AAOT
obs_id
marine plankton metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
0.09
µmol/L
phosphate
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
31.97
psu
salinity
Seabird SBE 911plus CTD system
salinity method
Niskin
sample collection device
WaSOP 1
sample material processing
DeepFreezer No2, second shelf, drawer 1
sample storage location
-78
°C
sample storage temperature
marine plankton metagenome
scientific_name
14.6
ºC
sea subsurface temperature
Seabird SBE 911plus CTD system
sea subsurface temperature method
25.48
ºC
sea surface temperature
Seabird SBE 911plus CTD system
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220622
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
4.73
µmol/L
silicate
3
µm
size-fraction lower threshold
200
µm
size-fraction upper threshold
EMOBON_AAOT_Wa_210622_3um_2
source material identifiers
sample
status
37.49
psu
subsurface salinity
Seabird SBE 911plus CTD system
subsurface salinity method
metag
target gene
metag
target subfragment
high
tidal stage
high_tide
tidal stage information
EMOBON_AAOT_Wa_2
title
17
m
total depth of water column
1.48
FTU
turbidity