2024-11-11
ENA first public
ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20569018
SRA accession
EMOBON00037
Submitter Id
10
L
amount or size of sample collected
marine biome [ENVO:00000447];estuarine biome [ENVO:01000020]
broad-scale environmental context
2.29
µg/L
chlorophyll
HPLC (Alliance HPLC System) following the method described by Zapata et al. 2000
chlorophyll method
2021-08-24
collection date
46.4582
mS/cm
conductivity
EXO2 multiparameter sonde
conductivity method
1
m
depth
EMOBON metagenome water sample from station PiEGetxo collected on 2021-08-24 size fraction 0.2-3um
description
44.7
ng/µl
dna concentration
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
environmental medium
Spain
geographic location (country and/or sea)
43.338583
DD
geographic location (latitude)
Abra de Bilbao
geographic location (locality)
-3.014639
DD
geographic location (longitude)
North Atlantic Ocean
geographic location (ocean)
Bay of Biscay
geographic location (region and locality)
52669421
library reads sequenced
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];harbor[ENVO:00000463]
local environmental context
DDC_AAAAOSDA_1_HMNJKDSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
PiEGetxo
obs_id
marine plankton metagenome
organism
7.8
pH
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
EXO2 multiparameter sonde
ph method
B-caroten: 0.04;α-caroten 0.005;chlorophyll b: 0.224;Lutein: 0.009;Zeaxanthin: 0.016;Diatoxanthin: 0;Aloxanthin: 0.038;Anteraxanthin: 0;Dinoxanthin: 0;Diadinoxanthin: 0.106;Hfu: 0.032;Violaxanthin: 0;Neoxanthin: 0;Prasinoxanthin: 0;Fucoxanthin: 0.278;Bfu: 0;Peridinin: 0.289;chlorophyll C1: 0.04;chlorophyll C2: 0.236
µg/L
pigments
HPLC (Alliance HPLC System) following the method described by Zapata et al. 2000
pigments method
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
33.16
psu
salinity
EXO2 multiparameter sonde
salinity method
Niskin
sample collection device
WaSOP 1
sample material processing
BBEBB, freezer 12
sample storage location
-80
°C
sample storage temperature
marine plankton metagenome
scientific_name
20.73
ºC
sea subsurface temperature
EXO2 multiparameter sonde
sea subsurface temperature method
20.73
ºC
sea surface temperature
EXO2 multiparameter sonde
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220712
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
0.2
µm
size-fraction lower threshold
3
µm
size-fraction upper threshold
EMOBON_PiEGetxo_Wa_210824_0.2um_1
source material identifiers
sample
status
33.16
psu
subsurface salinity
EXO2 multiparameter sonde
subsurface salinity method
metag
target gene
metag
target subfragment
low
tidal stage
low_tide
tidal stage information
EMOBON_PiEGetxo_Wa_3
title
10
m
total depth of water column
0
FTU
turbidity