2024-11-11
ENA first public
ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20568961
SRA accession
EMOBON00064
Submitter Id
5
L
amount or size of sample collected
Control
broad-scale environmental context
2021-08-31
collection date
0
m
depth
EMOBON metagenome water sample from station NRMCB collected on 2021-08-31 size fraction 3-200um
description
108
ng/µl
dna concentration
MilliQ water [ENVO:00003065]
environmental medium
missing: control sample
geographic location (country and/or sea)
40.800139
DD
geographic location (latitude)
Naples Gulf
geographic location (locality)
14.25
DD
geographic location (longitude)
Mediterranean Sea - Western Basin
geographic location (ocean)
Tyrrhenian Sea
geographic location (region and locality)
72214522
library reads sequenced
Negative
local environmental context
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_eu
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
NRMCB
obs_id
metagenome
organism
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:14
pcr conditions
metag
pcr primers
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
Niskin
sample collection device
Wa SOP1
sample material processing
Casotti minus80
sample storage location
-80
°C
sample storage temperature
metagenome
scientific_name
manual
sequence quality check
Batch1
sequence_batch
Batch1_220622
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
3
µm
size-fraction lower threshold
200
µm
size-fraction upper threshold
EMOBON_NRMCB_Wa_210831_3um_blank
source material identifiers
blank
status
metag
target gene
metag
target subfragment
no_tide
tidal stage information
EMOBON_NRMCB_Wa_27
title
75
m
total depth of water column