2024-11-11
ENA first public
ERC000011
ENA-CHECKLIST
École Polytechnique Fédérale de Lausanne (EPFL)
INSDC center name
public
INSDC status
ERS21882451
SRA accession
E-MTAB-14598:L3MBTL4
Submitter Id
ArrayExpress
broker name
not collected
collection date
Protocols: Input libraries were sequenced without any filtering. Eluted libraries (data from experiments) underwent selection based on TFs used in the SMILE-seq procedure. DNA libraries were collected after the SMiLE-seq procedure, purified and amplified via PCR. The amplified libraries were size selected using AMPureXP beads and amplified with i5 and i7 Nextera adapters to prepare for sequencing. Libraries were ordered as hand-mixed DNA oligos from IDT and synthesized via enzymatic reaction using DNA Polymerase I (Large Klenow Fragment).
description
not collected
geographic location (country and/or sea)
not applicable
isolate
synthetic construct
organism
synthetic construct
organism
synthetic construct
scientific_name
L3MBTL4
title