2024-11-11
ENA first public
ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20569030
SRA accession
EMOBON00031
Submitter Id
0.13
µmol/L
ammonium
Segmented flow analysis
ammonium method
5
L
amount or size of sample collected
marine biome [ENVO:00000447];marine pelagic biome [ENVO:01000023]
broad-scale environmental context
0.549
µg/L
chlorophyll
Chlorophyll extraction
chlorophyll method
2021-08-25
collection date
44.63
mS/cm
conductivity
SBE_37ODO
conductivity method
1
m
depth
EMOBON metagenome water sample from station EMT21 collected on 2021-08-25 size fraction 3-200um
description
7.439
µmol/kg
dissolved oxygen
SBE_37ODO
dissolved oxygen method
3.1
ng/µl
dna concentration
seawater [ENVO:00002149]; coastal sea water [ENVO:00002150]; surface water [ENVO:00002042]
environmental medium
Spain
geographic location (country and/or sea)
42.201944
DD
geographic location (latitude)
Vigo Seamount
geographic location (locality)
-8.7985
DD
geographic location (longitude)
Atlantic Ocean
geographic location (ocean)
North Atlantic Ocean
geographic location (region and locality)
95566929
library reads sequenced
marine pelagic feature [ENVO:01000044];marine water body [ENVO:00001999];costal water body [ENVO:02000049]
local environmental context
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_eu
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
mock_pr
0.23
µmol/L
nitrate
Segmented flow analysis
nitrate method
0.07
µmol/L
nitrite
Segmented flow analysis
nitrite method
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
EMT21
obs_id
marine plankton metagenome
organism
8.21
pH
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
Hach probe
ph method
0.13
µmol/L
phosphate
Segmented flow analysis
phosphate method
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
35.08
psu
salinity
Hach probe
salinity method
Niskin
sample collection device
WaSOP 1
sample material processing
cryopreservation room
sample storage location
-80
°C
sample storage temperature
marine plankton metagenome
scientific_name
16.21
ºC
sea subsurface temperature
SBE_37ODO
sea subsurface temperature method
17
ºC
sea surface temperature
Hach probe
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220622
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
3
µm
size-fraction lower threshold
200
µm
size-fraction upper threshold
EMOBON_EMT21_Wa_210825_3um_1
source material identifiers
sample
status
35.46
psu
subsurface salinity
SBE_37ODO
subsurface salinity method
metag
target gene
metag
target subfragment
low
tidal stage
ebb_tide
tidal stage information
EMOBON_EMT21_Wa_1
title
5
m
total depth of water column