2024-11-11
ENA first public
ERC000024
ENA-CHECKLIST
European Marine Biological Resource Centre
INSDC center name
public
INSDC status
ERS20568946
SRA accession
EMOBON00042
Submitter Id
5
L
amount or size of sample collected
marine biome [ENVO:00000447]
broad-scale environmental context
21.69
µg/L
chlorophyll
Fluorometer 10-AU
chlorophyll method
2021-08-05
collection date
0.2
m
depth
EMOBON metagenome water sample from station RFormosa collected on 2021-08-05 size fraction 3-200um
description
271.875
µmol/kg
dissolved oxygen
YSI ProSolo Digital
dissolved oxygen method
19.95
ng/µl
dna concentration
lagoon [ENVO_00000038];marine water body [ENVO:00001999]; surface water [ENVO:00002042]
environmental medium
Portugal
geographic location (country and/or sea)
37.005639
DD
geographic location (latitude)
Ria Formosa
geographic location (locality)
-7.96925
DD
geographic location (longitude)
Atlantic Ocean
geographic location (ocean)
North Atlantic Ocean
geographic location (region and locality)
86019436
library reads sequenced
lagoon [ENVO_00000038];marine water body [ENVO:00001999]
local environmental context
DDC_AAABOSDA_4_HMGW5DSX3.UDI213
mock_eu
DDC_AAAAOSDA_4_HMGW5DSX3.UDI201
mock_pr
metag
nucleic acid amplification
https://www.protocols.io/view/cryogrinding-protocol-mecanic-lysis-of-planktonic-n92ldyq88l5b/v1; https://www.protocols.io/view/rna-dna-extraction-from-plankton-natural-samples-u-eq2lynz2qvx9/v1; https://www.fishersci.com/shop/products/nucleospin-rna-ii-740955-250/NC0707522; https://www.mn-net.com/lysis-buffer-ra1-740961
nucleic acid extraction
RFormosa
obs_id
marine plankton metagenome
organism
8.242
pH
DNA Polymerase:taq Q5;NoPCRs:2;PCRvolume:30;Nocycles:10
pcr conditions
metag
pcr primers
Thermo scientific  orion star A221
ph method
0.998766
atm
pressure
YSI ProSolo Digital
pressure method
EMOBON
project name
Prod_ILL_BqADN_NEBUII_151_v4; Bq NEB Next Ultra II
relevant standard operating procedures
38.03
psu
salinity
YSI ProSolo Digital
salinity method
bucket
sample collection device
WaSOP 1
sample material processing
Ed7, room 1.10, freezer #300387-118
sample storage location
-80
°C
sample storage temperature
marine plankton metagenome
scientific_name
26.3
ºC
sea subsurface temperature
Thermo scientific  orion star A221
sea subsurface temperature method
26.3
ºC
sea surface temperature
Thermo scientific  orion star A221
sea surface temperature method
manual
sequence quality check
Batch1
sequence_batch
Batch1_220622
sequence_batch_rundate
Illumina NovaSeq 6000
sequencing method
3
µm
size-fraction lower threshold
200
µm
size-fraction upper threshold
EMOBON_RFormosa_Wa_210805_3um_2
source material identifiers
sample
status
38.03
psu
subsurface salinity
YSI ProSolo Digital
subsurface salinity method
metag
target gene
metag
target subfragment
low
tidal stage
low_tide
tidal stage information
EMOBON_RFormosa_Wa_2
title
1
m
total depth of water column