2024-11-16
ENA first public
ERC000015
ENA-CHECKLIST
WASHINGTON UNIVERSITY SCHOOL OF MEDICINE
INSDC center name
public
INSDC status
ERS22144701
SRA accession
23960
Submitter Id
unknown
broad-scale environmental context
2015-09-07
collection date
human
common name
healthy, HIV-1-uninfected
description
stool
environmental medium
USA
geographic location (country and/or sea)
38.7946
DD
geographic location (latitude)
-106.5348
DD
geographic location (longitude)
unknown
geographic location (region and locality)
23
years
host age
ASIAN
host race
female
host sex
685
host subject id
16S rDNA sequencing
library construction method
17506
library reads sequenced
unknown
local environmental context
PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA, with the reverse PCR primer barcoded with a 12-base error-correcting Golay code to facilitate multiplexing and used to amplify the V4 region of the bacterial 16S gene  Caporaso, J.G. et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample, Proc. Natl. Acad. Sci. U.S.A. 108 (supplement_1) 4516-4522, https://doi.org/10.1073/pnas.1000080107 (2011).
nucleic acid amplification
Phenol:chloroform
nucleic acid extraction
Homo sapiens
organism
APPROACH Microbiome
project name
200 mg stool
g
sample volume or weight for DNA extraction
Homo sapiens
scientific_name
MiSeq 2 _ 250 paired-end
sequencing method
HIV-V-A004-HA004-0685
title