2024-11-18
ENA first public
ERC000011
ENA-CHECKLIST
The Second Affiliated Hospital of Suzhou University
INSDC center name
public
INSDC status
ERS21345733
SRA accession
E-MTAB-14553:FIP_B
Submitter Id
ArrayExpress
broker name
not collected
collection date
brewer's yeast
common name
Protocols: Cells were grown in YPD broth at 30˚C from OD600=0.01 till OD600=1.0. Pellets of 5ml cell culture were obtained by centrifuging at maximum speed for 1min. the pellets were fast frozen in liquid nitrogen. RNA was extracted using the TRIzol method following the manufacturer's protocol. Cell pellets were transferred from liquid nitrogen into the 2ml tub containing 1.5ml Trizol reagent. The mix was shared for 3min, and kept for 5in at room temperature, then centrifuged at 10,000xg for 5min at 4˚C. The supernatant was added 200µl of chloroform/isoamyl alcohol (24:1) with 1ml lysis reagents. After centrifuged at 10,000g for 10min at 4˚C, the supernatant was transferred into another new tube with equal volume of isopropanol and put in the refrigerator at -20˚C for 1h. After centrifuged at 13600xg for 20min at 4˚C, the supernatant was precipitated by 1ml of 75% ethanol and let dry for 3-5min. The RNA pellet was dissolved with 30-100µl of DEPC water. The concentration of the extracted RNA was determined using a Nanodrop system (NanoDrop, Madison, USA), and the integrity of the RNA was examined by the RNA integrity number (RIN) using an Agilent 2100 bioanalyzer (Agilent, Santa Clara, USA) xxx
description
not collected
geographic location (country and/or sea)
not applicable
isolate
Saccharomyces cerevisiae
organism
Saccharomyces cerevisiae
organism
whole organism
organism part
Fluconazole induced petite
phenotype
Saccharomyces cerevisiae
scientific_name
S288c
strain
FIP_B
title