2024-11-18
ENA first public
ERC000011
ENA-CHECKLIST
Josep Carrers Leukemia Research Institute
INSDC center name
public
INSDC status
ERS19301980
SRA accession
E-MTAB-14046:SG_48
Submitter Id
ArrayExpress
broker name
ReNcell VM Human Neural Progenitor
cell_line
not collected
collection date
human
common name
Protocols: Samples were collected from cell cultures maintained under standard growing conditions for neural progenitor cells. Cells were pelleted and total RNA extraction was carried out with the Maxwell (Promega) technology.  Firstly, ribosomal RNA was removed by rRNA removal kit, and rRNA free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the rRNA-depleted RNA and performing procedures as follow. Briefly, after fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized and dUTPs were replaced with dTTPs in the reaction buffer. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.
description
Rett syndrome
disease
MECP2 mutation R133C
genotype
not collected
geographic location (country and/or sea)
not applicable
isolate
Homo sapiens
organism
Homo sapiens
organism
Homo sapiens
scientific_name
SG_48
title